Application of Src kinase inhibitor in preparing medicaments for treating acute kidney injury

A technology of acute kidney injury and kinase inhibitors, applied in the field of biology, can solve the problems of poor acute kidney injury and other problems, achieve the effect of alleviating renal pathological changes, inhibiting cell death, and achieving remarkable technological progress

Inactive Publication Date: 2017-07-07
SHANGHAI EAST HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Aiming at the above technical problems in the prior art, the present invention provides a use of a Src kinase inhibitor in the preparation of a drug for the treatment of acute kidney injury, said Src kinase inhibitor in the preparation of a drug for the treatment of acute kidney injury The purpose of the invention is to solve the technical problem that the effect of the medicine in the prior art on acute kidney injury is not good

Method used

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  • Application of Src kinase inhibitor in preparing medicaments for treating acute kidney injury
  • Application of Src kinase inhibitor in preparing medicaments for treating acute kidney injury
  • Application of Src kinase inhibitor in preparing medicaments for treating acute kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1.1 Materials and methods

[0038] (1) Reagents and antibodies

[0039] p-STAT3, STAT3, p-Src, Src, p-NF-kBp65, NF-kBp65, cleaved-caspase3, cleaved-PARP antibodies were all purchased from Cell Signaling Technology Company. E-cadherin, CD68, MCP-1, Claudin1, claudin4, MMP-2, MMP-9 and GAPDH antibodies were purchased from Santa Cruz. TUNEL kit was purchased from Roche LifeScience. Neutrophil gelatinase-associated apolipoprotein (NGAL) antibody was purchased from R&D Systems. α-tubulin antibody and other reagents were purchased from Sigma.

[0040] (2) Renal function test

[0041]Serum creatinine (Scr) and blood urea nitrogen (BUN) concentration detection kits were purchased from Bio Vision and Sigma, respectively, and operated according to the instructions.

[0042] (3) Renal ischemia-reperfusion injury model and treatment groups

[0043] The mouse I / R model was established according to the method reported in the literature. Mice were anesthetized with ketamine (75...

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Abstract

The invention provides application of Src kinase inhibitor in preparing medicaments for treating acute kidney injury. Further, the acute kidney injury is caused by renal ischemia. Further, the Src kinase inhibitor is PP1 (4-Amino-5-(methylphenyl)-7-(t-butyl)pyrazolo-(3,4-d)pyrimidine). The invention proves that PP1 inhibition of Src can relieve the pathobiological change of kidney after I/R, so as to improve kidney function. The treatment mechanism of the Src kinase inhibitor includes procedures of inhibiting apoptosis of renal tubule cells, protecting multiple connexins and adhesive proteins, and inhibiting cell death and inflammation signal pathway.

Description

[0001] Technical field: [0002] The invention belongs to the field of biology and relates to a Src kinase, in particular to the use of a Src kinase inhibitor in the preparation of medicines for treating acute kidney injury. [0003] Background technique: [0004] Acute kidney injury (AKI) is a clinical emergency and critical illness caused by various reasons including ischemia-reperfusion (I / R). A large number of studies have proved that the mortality rate of AKI is as high as 40-60%, and there is still a lack of effective treatment methods. It is very important to explore the pathogenesis and therapeutic targets of acute kidney injury. [0005] In addition, AKI can progress to chronic kidney disease and eventually to end-stage renal disease. The molecular mechanism of AKI has not yet been fully clarified, and there is no treatment that changes the natural history of AKI. Therefore, elucidating the pathogenesis of AKI and finding new ways to treat it are necessary to improve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/519A61P13/12
CPCA61K31/519
Inventor 庄守纲
Owner SHANGHAI EAST HOSPITAL
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