A traditional Chinese medicine composition capable of inhibiting the proliferation of lung cancer cells and its application
A technology of lung cancer cells and compositions, which is applied in the direction of drug combinations, medical preparations containing active ingredients, and pharmaceutical formulas, which can solve problems such as leukopenia, weight loss, and damage to normal tissue cells, and achieve low toxic and side effects and low cost. cheap effect
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Embodiment 1
[0022] Embodiment 1 Chinese medicine composition
[0023] Traditional Chinese medicine composition formula 1: Epimedium 8g, Boluohui 4g, Magnolia officinalis 7g, Licorice 6g.
[0024] Chinese medicine composition formula 2: Epimedium 9g, Boluohui 8g, Magnolia officinalis 8g, and Licorice 7g.
[0025] Traditional Chinese medicine composition formula 3: Epimedium 10g, Boluohui 12g, Magnolia officinalis 9g, licorice 8g.
[0026] Chinese medicine composition formula 4: Epimedium 11g, Boluohui 16g, Magnolia officinalis 10g, Licorice 9g.
[0027] Traditional Chinese medicine composition formula 5: Epimedium 12g, Boluohui 20g, Magnolia officinalis 11g, Licorice 10g.
Embodiment 2
[0028] Embodiment 2 Chinese medicine composition is made liquid medicine
[0029] The traditional Chinese medicine composition of the five formulas in Example 1 was made into a medicinal solution, and the preparation method was as follows: using 50% alcohol extraction method to extract Epimedium, Boluohui, Magnolia officinalis and Glycyrrhizae for medicinal solution preparation, and rotary evaporation into The required concentration of liquid medicine, corresponding to formula 1-5, is formula 1 liquid, formula 2 liquid, formula 3 liquid, formula 4 liquid, formula 5 liquid.
Embodiment 3
[0030] Example 3 Preparation of Normal Fetal Rat Lung Epithelial Cells
[0031] Method: Pregnant Kunming female mice with 19 days of pregnancy were selected, and the fetal mice were taken out by cesarean section in a sterile environment. The normal mouse lung cells were taken out, and the lungs of the fetal mice were removed. Impurities were removed, and they were digested with trypsin and collagen fiber enzyme for many times. Obtain the mixed mouse lung cell (isolated and cultured by the Veterinary Medicine Research and Development Office of Southwest University Rongchang Campus) suspension, inoculate it in a culture bottle, and incubate at 37°C, 5% CO 2 Culture in an incubator, and observe the growth of the cells and the changes in their morphology.
[0032] Results: The pneumocytes obtained by cesarean-sectioned fetal mice were evenly scattered and distributed in cell flasks after digestion and separation by trypsin and collagen fibril enzymes, as shown in Fig. figure 1 Af...
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