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A kind of culture medium and propagation method of tissue culture rapid propagation of alum root blade

A tissue culture and culture medium technology, applied in the field of plant tissue culture, can solve the problems of not being able to meet the market demand, the leaves cannot be induced to sprout, and the selection of explants is single, so as to accelerate the promotion of improved varieties, high plant regeneration efficiency, and improve The effect of multiplier

Inactive Publication Date: 2019-03-01
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The conventional propagation method of alum root is branch propagation, the reproduction amount is limited, and affected by the season, it cannot meet the market demand
[0004] There are many reports on alum root tissue culture, but there are the following problems: 1) The selection of explants is single and limited, and the explants in related reports all choose "young stems with terminal buds or axillary buds" (Sun Guofeng, 2007; Zhang Zhihong , 2011; Chen Hong, 2011; Gao Yan, 2015), and also reported that "leaves cannot induce budding" (Wang Jing, 2012)
For explants, stem segments with terminal buds or axillary buds are selected. This destructive sampling not only obtains a small number of explants, but also leads to the destruction of the entire plant of the mother body, resulting in waste of materials; Hormones use different ratios of 6-BA and NAA, the induction rate of clustered buds is low and prone to vitrification, the value-added multiple of proliferation and growth is generally 8-9, and the production cycle is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for the rapid propagation of tissue culture of alum root blades, the following steps are carried out successively:

[0033] (1) The preparation of the culture medium, including the components of the basic medium and the culture medium at each stage of tissue culture, and the weight per liter:

[0034] 1) Basic medium: LS basic medium is used for cluster bud induction culture, proliferation, and strong seedling culture, and 1 / 2LS is used for rooting culture.

[0035] 2) Cluster bud induction medium: LS + 6-BA 2.0 mg / L + NAA 0.2 mg / L + sucrose 20g / L + agar 8g / L, pH5.6~5.8

[0036] 3) Proliferation and growth medium: LS + 6-BA 1.0 mg / L + NAA 0.1 mg / L + sucrose 25g / L + agar 5g / L, pH 5.6-5.8.

[0037] 4) The rooting medium is: 1 / 2LS + NAA0.5mg / L + sucrose 30g / L + agar 5g / L, pH5.6~5.8;

[0038] (2) Culture of alum root tissue culture seedlings

[0039] 1) Selection of explants: Select 1-2 year-old leaves of alum root with strong growth and no disease spots as expl...

Embodiment 2

[0047] In this example, the preparation of the medium in step (1), including the basic medium and the components of the medium at each stage of tissue culture, and the weight per liter are:

[0048] 2) Cluster bud induction medium: LS + 6-BA 3.0 mg / L + NAA 0.2 mg / L + sucrose 20g / L + agar 8g / L, pH5.6~5.8

[0049] 3) Proliferation and seedling growth medium: LS + 6-BA 0.5 mg / L + NAA 0.1 mg / L + sucrose 25g / L + agar 5g / L, pH 5.6-5.8.

[0050] 4) The rooting medium is: 1 / 2LS + NAA1.0mg / L + sucrose 30g / L + agar 5g / L, pH5.6~5.8;

[0051] All the other steps, technology are with embodiment 1.

Embodiment 3

[0053] In this example, the preparation of the medium in step (1), including the basic medium and the components of the medium at each stage of tissue culture, and the weight per liter are:

[0054] 2) Cluster bud induction medium: LS + 6-BA 2.0 mg / L + NAA 0.1 mg / L + sucrose 20g / L + agar 8g / L, pH5.6~5.8

[0055] 3) Proliferation and growth medium: LS + 6-BA 1.0 mg / L + NAA 0.2 mg / L + sucrose 25g / L + agar 5g / L, pH 5.6-5.8.

[0056] 4) The rooting medium is: 1 / 2LS + NAA1.5mg / L + sucrose 30g / L + agar 5g / L, pH5.6~5.8;

[0057] All the other steps, technology are with embodiment 1.

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PUM

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Abstract

The invention discloses a culture medium and a propagation method for tissue culture rapid propagation of leaves of heuchera micrantha. Through selection on an explants, selection on a basic culture medium and hormone combination in proper dosage, high-effective regeneration of a plant of the heuchera micrantha during the tissue culture is achieved. The propagation method is simple operation and low pollution rate, is high in success rate of regeneration of the plant, and is short in production period of grown-up seedlings.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium and a propagation method for rapid propagation of alum root leaf tissue culture. Background technique [0002] Alum root ( Heuchera micrantha ) also known as Coral Bell, Saxifragaceae Alumia, loves the sun, is shade-tolerant, extremely cold-resistant, can grow normally at a low temperature of minus 30°C, has rich leaf colors and changeable leaf shapes, and is an excellent shade cover for colorful leaves plant. It has high ornamental value, good quality, high economic benefits, broad prospects for development and utilization, and extremely promising market prospects. [0003] The conventional propagation method of alum root is branch propagation, the reproduction amount is limited, and affected by the season, it cannot meet the market demand. [0004] There are many reports on alum root tissue culture, but there are the following problems: 1) The s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 邹清成朱开元马广莹刘慧春史小华
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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