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Industrialized seedling production and artificial cultivation method of dryopteris fragrans

A technique for artificial cultivation of fern, which is applied in the directions of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., and can solve the impact of wild ferns, the easy death of ferns, and the destruction of ecological environment. and other problems to achieve the effect of reducing ineffective transpiration, shortening the growth cycle, and realizing factory production.

Active Publication Date: 2017-07-14
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the destruction of the ecological environment and the discovery of its medicinal value by humans, the resources of wild hair fern are seriously affected
The process cycle of inducing sporophytes by ordinary tissue culture and soil culture is long, and wild D. chrysalis is easy to die in the induction process. Therefore, how to utilize artificial cultivation techniques to cultivate D. edulis with high yield has become a problem in the prior art. Technical problem, there is no related report in the prior art

Method used

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  • Industrialized seedling production and artificial cultivation method of dryopteris fragrans
  • Industrialized seedling production and artificial cultivation method of dryopteris fragrans
  • Industrialized seedling production and artificial cultivation method of dryopteris fragrans

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Embodiment 1

[0040] The present embodiment provides a kind of industrialized seedling cultivation and artificial cultivation method (tissue culture method) of Trichophyllum chrysalis, and the experience method is as follows: 1, the growth of gametophytes

[0041]Take the mature spores of Trichophylla spp., put them in a 1.5mL centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, and centrifuge at 4 000r / min for 1min to precipitate all the spores, and discard the supernatant. Then drop about 1.0mL of 0.1% HgCl into the centrifuge tube 2 solution, sterilized for 1 min, rinsed with sterile water for 4 to 5 times, and obtained a sterile spore suspension by the above-mentioned centrifugation method.

[0042] The solid medium adopts 1 / 2MS, the concentration of sucrose is 3.0%, and the concentration of agar is 0.7%-0.8%. The medium was divided into Erlenmeyer flasks and sterilized under high pressure (121°C, 20min) for later use.

[0043] Liquid medium culture is ...

Embodiment 2

[0052] The present embodiment provides a kind of industrialized seedling raising and artificial cultivation method (soil cultivation method) of Trichophyllum chrysalis, and the experience method is as follows: 1, the growth of gametophytes

[0053] Take the mature spores of Trichophylla spp., put them in a 1.5mL centrifuge tube, drop them into sterile water, oscillate fully to make a suspension, and centrifuge at 4 000r / min for 1min to precipitate all the spores, and discard the supernatant. Then drop about 1.0mL of 0.1% HgCl into the centrifuge tube 2 solution, sterilized for 1 min, rinsed with sterile water for 4 to 5 times, and obtained a sterile spore suspension by the above-mentioned centrifugation method.

[0054] The soil matrix is ​​made of peat soil and vermiculite that have been autoclaved (121°C, 20min) at a volume ratio of 3:1, and packed in plastic bowls for later use.

[0055] Inoculate the obtained spore suspension into a plastic pot filled with soil substrate ...

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Abstract

The invention discloses a high-yield artificial cultivation method for sporophytes of dryopteris fragrans and belongs to the technical field of cultivation of the dryopteris fragrans. The method includes the steps of: 1) processing mature spores of the dryopteris fragrans into a sterile spore suspension liquid, and inoculating the sterile spore suspension liquid on a liquid culture medium, performing dark treatment to synchronously germinate the spores and culture the spores; 2) during a gametophyte stage, inoculating the spores on a solid culture medium and continuously culturing the spores to obtain gametophytes of the dryopteris fragrans, which have excellent growth status; and 3) moving out and washing the gametophytes of the dryopteris fragrans, and moving the gametophytes to a soil matrix, in which gametophytes of ceratopteris thalictroides are cultured, for cultivation to obtain mature sporophytes of the dryopteris fragrans. The method overcomes the problems that a process of inducing the sporophores through tissue culture and soil culture is long in period and wild dryopteris fragrans is liable to die during the induction process. The method is suitable for industrialized production of the sporophytes of dryopteris fragrans and provides a powerful technology support for large-scale production of the sporophytes of dryopteris fragrans.

Description

technical field [0001] The invention relates to an industrialized seedling raising and artificial cultivation method of D. edulis, belonging to the technical field of D. edulis cultivation. Background technique [0002] Dryopteris fragrans (L.) Schott is a wild perennial fern belonging to the genus Dryopteris fragrans (L.) Schott. The fresh leaves are crushed and have fragrance, also known as Dryopteris fragrans (L.) Schott. Unlike most ferns that grow in humid and high-temperature environments, the growth environment of Dermascens is very special. It is born in talc slopes, lava crevices and volcanic magma crevices formed after volcanic eruptions. In the world, the hair fern is distributed in China, Japan, North Korea, Mongolia, Russia and North America, in the alpine zone with an altitude of about 1000-1700m. In China, Trichofern is mainly distributed in the Wudalianchi area of ​​Heilongjiang Province. [0003] Trichofern can tolerate low temperature and can still surviv...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 常缨卜志刚陶文青陈玲玲王琪
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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