Muscodor sp.W-S-41 and application thereof

A technology of musk mold and use, which is applied in the field of microorganisms, can solve the problems of different activity effects, etc., and achieve the effects of not destroying the ecological balance, great application potential, and not polluting the environment

Inactive Publication Date: 2017-07-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Muscodor sp., previously named as aerogenes, is a type of endophytic fungus belonging to the family Cycloceraceae. It was first isolated from cinnamon by Worapong et al. (2001). People use its unique morphology, Physiological and biochemical characteristics and ribosomal RNA sequences were used to find and identify new endophytic fungi of the genus Muscarina. Its distinctive feature is the ability to produce volatile organic compounds (volatile organic compounds, referred to as VOCs). These VOCs have a wide range of Biological activity, can inhibit or kill many pathogenic fungi, pathogenic bacteria and some insects, but different strains have different active effects

Method used

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  • Muscodor sp.W-S-41 and application thereof
  • Muscodor sp.W-S-41 and application thereof
  • Muscodor sp.W-S-41 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Isolation and acquisition of Muscodor sp.W-S-41 strain

[0025] Collect the weed grass in the wild state from Naban River, Yunnan, my country, rinse the collected weed grass with tap water, disinfect the weed grass leaf sheath in 75% (v / v) alcohol solution for 30 seconds, and then disinfect it in 1% (v / v) ) in sodium hypochlorite solution for 10 minutes and rinsed with sterile water for 3 times. Cut the roots into about 0.6 cm long fragments with a sterile razor blade, and place them in a -1 Ampicillin and 50 μg·ml -1 Streptomycin 2% malt juice agar medium (MEA) plate, 25 ℃ dark culture, after mycelium grows, the top of the mycelium is transplanted to PDA medium; isolated white colony, spreading radially, Culture in the dark at 25°C, inoculate three times in succession on PDA medium, confirm that it is a single colony, no other fungi or bacteria grow together, it is considered to be a pure culture, named Muscodor sp.W-S-41.

[0026] The strain is densely white on t...

Embodiment 5

[0081] Embodiment five, musk mold strain Muscodor sp.W-S-41 is to the bacteriostasis of ultimate Pythium ZAU22 (Pythium ultimum)

[0082] In this experiment, the ultimate Pythium ZAU22 (Pythium ultimum) was selected as the pathogenic bacteria, and the two-partition plate confrontation culture method was used to measure the inhibitory effect of the volatile gas of the musk mold strain Muscodor sp.W-S-41 on the ultimate Pythium ZAU22 (Pythium ultimum). Musk mold strains Muscodor sp.W-S-41, Muscodor sp.ZJLQ023, Muscodor sp.ZJLQ024 and Muscodor sp.ZJLQ070 were tested together, and the rest were equal to Experiment 1; the results in Table 6 were obtained as follows:

[0083] Table 6: Muscodor sp.W-S-41, Muscodor sp.ZJLQ023, Muscodorsp.ZJLQ024 and Muscodor sp.ZJLQ070 and the ultimate Pythium ZAU22 (Pythium ultimum) two separate plate confrontation culture results

[0084]

[0085]Pythium ultima was originally isolated from rotting cress seedlings in the UK, and it has been report...

Embodiment 2

[0089] Embodiment 2, musk mold Muscodor sp.W-S-41 inoculum preparation

[0090] Based on the fact that the muscodor sp. W-S-41 described in the present invention has a high-efficiency and broad-spectrum fungicidal effect, the present invention also relates to an inoculant comprising the muscodor sp. W-S-41. The bacterial agent of the present invention refers to a live bacterial preparation made from beneficial microorganisms, including biological preparations, biological fertilizers, biological pesticides, active carrier preparations, etc., including the musk mold strain Muscodor sp.W-S- 41 or various single products or combinations of metabolites of Muscodor sp. W-S-41. The active carrier preparation of this embodiment includes a solid carrier, a culture medium, a stabilizer and live bacteria.

[0091] The present invention prepares the method for strain Muscodor sp.W-S-41 active carrier preparation, the method comprises (1) the preparation of Muscodor sp.W-S-41 seed culture...

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Abstract

The invention discloses muscodor sp.W-S-41, the preservation number of which is CCTCC NO: M2016759. The muscodor sp.W-S-41 can inhibit plant pathogenic fungi comprising Botrytis cinerea ZAU10, Pestalotiopsis sp. ZAU20, Fusarium sp. ZAU 28, Penicillium digitatum KH08, Pythium ultimum ZAU22 and the like.

Description

technical field [0001] The invention relates to the field of microorganisms, and provides an endophytic fungus, specifically a musk mold strain, which has good antibacterial activity, especially the activity of inhibiting the growth of plant pathogenic fungi. Background technique [0002] Muscodor sp., previously named as aerogenes, is a type of endophytic fungus belonging to the family Cycloceraceae. It was first isolated from cinnamon by Worapong et al. (2001). People use its unique morphology, Physiological and biochemical characteristics and ribosomal RNA sequences were used to find and identify new endophytic fungi of the genus Muscarina. Its distinctive feature is the ability to produce volatile organic compounds (volatile organic compounds, referred to as VOCs). These VOCs have a wide range of Biological activity can inhibit or kill many pathogenic fungi, pathogenic bacteria and some insects, but different strains have different active effects. Because musk mold can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 章初龙冯晓晓毛黎娟林福呈
Owner ZHEJIANG UNIV
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