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A kit for detecting and identifying dry eye syndrome
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A dry eye syndrome and kit technology, applied in the field of medicine, can solve the problems of negative prompts and low sensitivity, and achieve the effect of eliminating subjective discomfort, high sensitivity and good specificity
Active Publication Date: 2020-06-09
PEKING UNIV THIRD HOSPITAL
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Positive MMP-9 indicates the severity of inflammation and has a good correlation with moderate to severe dry eye, but negative indicates that the sensitivity is not high
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Embodiment 1
[0049] IL-6, IL-23 concentration and dry eye syndrome in the tear fluid of embodiment 1
[0050] 1. Sample source
[0051] SS dry eye group: 15 cases (30 eyes) of SS dry eye patients;
[0053] Normal control group: 15 healthy volunteers (30 eyes).
[0054] All patients signed informed consent before entering the study, and the study was approved by the Biomedical Ethics Committee of Peking University, batch number: IRB00001052-08081.
[0055] 2. Tear collection
[0056] Place the capillary in the patient's tear river, carefully collect 1 μl of tear fluid, and try to avoid irritating the patient's eyeballs to obtain the underlying tear fluid. The collected tear fluid was quickly transferred to the EP tube, and 9 μl of 1% BSA should be added to the EP tube in advance. Seal the EP tube around with a parafilm and store it in a -80°C refrigerator for later use.
[0057] 3. Luminex Multiplex Immunobe...
Embodiment 2
[0070] Embodiment 2 detects dry eye syndrome based on IL-6
[0071]After screening the cytokine IL-6 in tears, 40pg / ml is used as the cut-off value. If the IL-6 content in the sample is higher than the cut-off value, it is judged as dry eye syndrome. If it is lower than the cut-off value, it is judged as normal and not suffering from Dry eye syndrome, Sensitivity and specificity of IL-6-based diagnostic kits and methods in the diagnosis of dry eye, compared with the value of the InflammaDry MMP-9 tester (eg Sambursky R, Davitt WF 3rd, Latkany R, et al al.Sensitivity and specificity of a point-of-care matrix metalloproteinase 9immunoassay for diagnosinginflammation related to dry eye.JAMA Ophthalmol.2013Jan; 131(1):24-8), the results are shown in Table 1 and Table 2.
[0072] The results of dry eye identification based on IL-6 detection in the samples in Table 1
[0078] Example 3 dry eye classification and IL-17A
[0079] The cytokines in the tears of patients with chronic dry eye were detected by Luminex multiplex immunobeads, and the test results are shown in Table 3.
[0080] Table 3 IL-6, IL-17A detection results
[0081]
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Abstract
A kit for detecting and identifying a dry eye syndrome is disclosed. The kit includes a compound used for detecting the interleukin 6 (IL-6) protein level. According to the kit, the IL-6 level in a detected sample is adopted as an early diagnosis indication of the dry eye syndrome, detection is rapid, simple and convenient, the lost is low, sensitivity is high, specificity is good and the kit is safe and reliable. The kit can achieve early diagnosis on whether a patient is affected with the dry eye syndrome or not, excludes subjective discomforts caused by psychological and mental disorders, can prompt an inherent reason of the dry eye syndrome (such as SS dry eye syndrome), provides dry eye syndrome graded treatment with objective bases and provides an objective evaluation system for development of novel medicine treating the dry eye syndrome.
Description
technical field [0001] The invention relates to the technical field of medicine, in particular to a kit for detecting and identifying dry eye disease. Background technique [0002] Dry eye syndrome, also known as keratoconjunctivitis sicca, is a multifactorial disorder of the tear fluid and ocular surface that causes symptoms of discomfort, visual disturbance, and tear film instability with potential damage to the ocular surface, and This is accompanied by increased permeability of the tear film and inflammation of the ocular surface. Dry eye is considered a disorder of the lacrimal functional unit, a complete system of the lacrimal gland, ocular surface (cornea, conjunctiva, and meibomian glands), and eyelids, and the sensory and motor nerves connecting them. The lacrimal functional unit controls the major components of the tear film and responds to environmental, endocrinological, and cortical influences in a regulated manner. The function of this unit is to maintain the...
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