Application of polymethoxylflavone in preparing medicine for treating melanoma

A technology for polymethoxyflavones and melanoma, which is applied to the application field of polymethoxyflavones in the preparation of medicines for treating melanoma, can solve problems such as unclear etiology, and achieves wide source of raw materials, improved inhibitory effect, and improved inhibition of melanoma. effect of growth

Pending Publication Date: 2017-08-04
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For acral melanoma in Asia and Africa, the etiology is currently unknown

Method used

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  • Application of polymethoxylflavone in preparing medicine for treating melanoma
  • Application of polymethoxylflavone in preparing medicine for treating melanoma
  • Application of polymethoxylflavone in preparing medicine for treating melanoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Effects of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on the growth and cell cycle distribution of human melanoma cell lines A2058 and A375 cells

[0013] The cultured cells are human melanoma cell lines A2058 and A375 cells, the medium is DMEM medium, containing 100U / ml penicillin and 100μg / ml streptomycin, 10% fetal bovine serum, the culture temperature is 37°C, the concentration of carbon dioxide 5%. Add 100 μl of 2% DMSO solution to each liter of DMEM medium; add 100 μl of 3.125 μmol of 5-hydroxy-3,6,7,8,3',4'-hexamethanone to each liter of DMEM medium 2% DMSO solution of oxyflavone; add 100 μl of 2% DMSO solution containing 6.25 μmol of 5-hydroxyl-3,6,7,8,3',4'-hexamethoxyflavone in every liter of DMEM medium; Add 100 μl of 2% DMSO solution containing 12.5 μmol of 5-hydroxy-3,6,7,8,3’,4’-hexamethoxyflavone to each liter of DMEM medium; add 100 μl to each liter of DMEM medium 2% DMSO solution containing 25.0 μmol of 5-hydroxy-3,6,7,8,3’,4’-hexamethoxyfl...

Embodiment 2

[0015] Example 2: Effect of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone on tumor growth in C57bl / 6 mice induced by human melanoma cell line A375 cells

[0016] C57bl / 6 mice were divided into 2 groups with 8 mice in each group. According to the time interval of feeding once every 2 days, 0.4ml of DMSO solution with a mass concentration of 2%, and 0.4ml of 0.5μmol 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone were fed respectively. DMSO solution, inoculated human melanoma cell line A375 cells 28 days later, sacrificed the mice, and detected the size of the tumor tissue in the C57bl / 6 mice of each group ( image 3 ). The experimental results show that the tumor volume of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone is 890mm3, the tumor volume of 2% DMSO solution treatment group is 2450mm3, 5-hydroxy-3 , The tumor volume of the 6,7,8,3',4'-hexamethoxyflavone treatment group was significantly smaller than that of the 2% DMSO solution treatment group, indicating that it has strong anti-...

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Abstract

The invention relates to polymethoxyflavone for treating melanoma. The effective ingredients of the polymethoxylflavone comprise one or multiple of hesperetin, nobiletin, sinensetin, 5-hydroxy-3,6,7,3',4'-pentamethoxylflavone, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, and 5-hydroxy-6,7,4'-trimethoxylflavone. The polymethoxylflavone has the advantages that after the polymethoxylflavone acts for 48h, the survival rates of melanoma cell lines A2058 and A375 are respectively 42% and 26%; compared with a contrast group, the inhibition action of the polymethoxylflavone on the tumor growth in a C57b1/6 rat inoculated with the melanoma cell lines A375 is increased by 2.75 times.

Description

technical field [0001] The invention relates to the application of polymethoxylated flavones in the preparation of medicines for treating melanoma. Background technique [0002] Melanoma is a malignant tumor formed after melanocytes in the skin, mucous membrane, and choroid of the eye become cancerous. It is the most malignant tumor among skin tumors and is prone to distant metastasis. The primary sites of melanoma are mostly the soles of the feet, toes, the ends of the fingers, and under the nails. For Caucasians such as Europe and the United States, the main factor for the formation of melanoma is the formation of DNA mutations caused when the skin is exposed to ultraviolet rays. For acral melanoma in Asia and Africa, the etiology is still unclear. The therapeutic effect of melanoma is related to the stage of melanoma. The 5-year survival rates of patients with stage I, stage II, stage III and stage IV melanoma are 94%, 44%, 38%, and 4.6%, respectively. Early-stage mela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 李士明杨谷良龙涛
Owner HUANGGANG NORMAL UNIV
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