Efficient multi-functional compound bacterial fertilizer capable of quickly decomposing straws and preparation method of efficient multi-functional compound bacterial fertilizer
A technology of decomposing straw and compound bacterial fertilizer, which is applied in the field of high-efficiency multifunctional compound bacterial fertilizer and its preparation, can solve the problems of high price, damage to soil structure, and single function, so as to reduce crop diseases and insect pests, improve soil structure, and increase crop yield Effect
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[0031] Prepare each culture medium first:
[0032] The formula of the bran culture medium of Sporiosporium thermophila: bran 800g, soybean flour 200g, K 2 HPO 4 0.5g, MgSO 4 0.5g, 1000ml distilled water, adjust the pH value to 6-8. The above medium together with the prepared LB medium, nitrogen-fixing bacteria medium and beef extract peptone medium were steam sterilized at 121°C for 20 minutes at 0.1 MPa for later use.
[0033] then follow figure 1 As shown in the process, the high-efficiency multi-functional compound bacterial fertilizer for rapidly decomposing straw is prepared through the following steps;
[0034] Preparation of liquid bacterial fertilizer:
[0035] (11) Cultivation: Inoculate Vibrio cellulosus in LB medium, shake culture at 28°C for 48-72h; Sporiosporium thermophile inoculate in bran medium, culture at constant temperature at 45°C for 48-72h, collect spores; Pseudomonas Bacteria were inoculated in LB medium and cultured at a constant temperature of 2...
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