Real-time fluorescent PCR assay for detection of goat-derived components in food and feed using single-copy nuclear genes

Abstract

The invention discloses real-time fluorescent PCR (polymerase chain reaction) detection method for detecting goat-derived ingredients in foods and feeds through single-copy nuclear genes; the method comprises the steps of first, using DNA of a sample under detection as a template to perform fluorescent quantitative PCR amplification to obtain a PCR amplification product; second, detecting a fluorescent signal of the amplification product; third, judging whether the sample contains goat-derived ingredients or not according to Ct value in the detection results, wherein the reaction system for PCR amplification contains a specific primer pair for amplifying goat-derived ingredients and a specific probe for goat-derived ingredients. The specific primer pair and probe for the real-time fluorescent PCR amplification of goat-derived ingredients have good specificity and high sensitivity, the quantitative detection method is provided for the quick and accurate detection of whether foods and feeds contain goat-derived ingredients, and the method has a promising application prospect.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a real-time fluorescent PCR detection method for detecting goat-derived components in food and feed by using a single-copy nuclear gene. Background technique [0002] Since it has always been difficult to distinguish goat meat from sheep meat, goats and sheep are usually not distinguished in many food and feed tests, but only whether they contain sheep ingredients, which has buried an invisible impact on food safety. Hidden dangers also give some criminals an opportunity to adulterate for profit. At the beginning of 2013, Sweden, the United Kingdom and other European countries were involved in the "horse meat scandal" scandal, which aroused the attention of various countries on the adulteration of meat and meat products. [0003] In my country, the issue of "adulteration" has always been the focus of consumer complaints and a hot spot of social concern. Driven by profi...

AI Technical Summary

Problems solved by technology

[0009] The primary purpose of the present invention is to provide a real-time fluorescent PCR detection method for goat-derived components in food and feed, so as to overcome the deficiencies in the prior art that are difficult to quantitatively detect and prone to false positive results.

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