A kind of induction method of dendrobium polyploid

A technology of polyploidy and Dendrobium, applied in the field of plant genetics and breeding, can solve problems such as heavy workload and complicated operating procedures

Active Publication Date: 2019-04-12
云南丰春坊生物科技有限公司 +1
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  • Abstract
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Problems solved by technology

However, under in vitro conditions, the problem of chromosome ploidy mosaicism is common in the treatment of materials such as clustered buds and protocorms. It is necessary to use adventitious bud induction technology to continuously segment mutant plant stems, leaves and other tissues to induce ploidy homozygosity. polyploid plants, the operation procedures are complicated and the workload is heavy

Method used

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  • A kind of induction method of dendrobium polyploid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The hybrid Dendrobium species (Dendrobium nobile × Dendrobium candidum) with no occurrence of diseases and insect pests and strong growth were selected as induced plants in the field.

[0017] When entering the flowering stage, the pollen blocks of Dendrobium nobile are used for pollination.

[0018] Samples were taken every 5 days after pollination, and the progress of meiosis and female gametophyte development of Dendrobium megaspore mother cells at different pollination times were microscopically observed. The observation results showed that 45 days after pollination, Dendrobium was mainly in the megaspore mother cell stage.

[0019] 45 days after pollination, the capsules were placed in a centrifuge tube filled with 30 mL of 60 μM Oryzalin aqueous solution and soaked for 48 hours. After the treatment, the capsules were taken out and washed with water to remove Oryzalin. Among them, the 60 μM Oryzalin aqueous solution was prepared in advance according to the fol...

Embodiment 2

[0023] The hybrid Dendrobium species (Dendrobium nobile × Dendrobium candidum) with no occurrence of diseases and insect pests and strong growth were selected as induced plants in the field.

[0024] When entering the flowering stage, the pollen blocks of Dendrobium nobile are used for pollination.

[0025] Samples were taken every 5 days after pollination, and the microscopic observation of the meiosis of Dendrobium megaspore mother cells and the development of female gametophytes at different pollination times showed that, 50 days after pollination, Dendrobium was mainly in the stage of megaspore mother cell meiosis .

[0026] 50 days after pollination, the capsules were placed in a centrifuge tube filled with 30 mL of 60 μM Oryzalin aqueous solution and soaked for 48 hours. After the treatment, the capsules were taken out and washed with water to remove Oryzalin. The preparation method of Oryzalin aqueous solution is identical with embodiment 1.

[0027] Harvesting ...

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Abstract

The invention discloses an induction method of Dendrobium polyploidy, comprising the steps of using 60-90 mu M of Oryzalin to soak pollinated Dendrobium megasporocytes and Dendrobium capsules in meiosis phase continuously for 48-72 hours, washing with clear water to remove Oryzalin, aseptically germinating seeds, identifying chromosome, and acquiring a polyploid plant from offspring. The chimera problem of Dendrobium polyploidy is solved, the problem that conventional triploid cultivation takes long time and is complex to operate is solved, and a triploid can be successfully cultivated conveniently within one growth period.

Description

technical field [0001] The invention relates to a method for inducing polyploidy in plants, in particular to a method for inducing polyploidy in Dendrobium nobile, belonging to the field of plant genetics and breeding. Background technique [0002] Polyploid refers to plants whose somatic cells have more than two chromosome sets. In Dendrobium nobile, polyploidy is obtained mainly by inducing chromosome doubling in somatic cells under in vitro culture conditions. Soaking the clustered buds of Dendrobium dendrobium with 0.03% colchicine for 24 hours can better obtain polyploidy (preliminary report on the induction of polyploidy in Dendrobium dendrobium, Yunnan Botanical Research 2005, 27(5): 552-556) . Among the colchicine solution soaked dendrobium bushy seedlings to induce chromosome doubling, 0.06% colchicine treatment for 12 hours had the best mutagenic effect (Polyploid Induction of Spring Dendrobium, Acta Horticultural Science, 2009, 36(9): 1381-1384) . The protocor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 董志渊杨斌马维思王晓云王馨杨丽英李林玉严世武徐志峰
Owner 云南丰春坊生物科技有限公司
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