Extraction and separation purification method for total alkaloids in zanthoxylum dissitum hemsl

A technology for separation and purification of total alkaloids, applied in the fields of plant/algae/fungus/moss components, pharmaceutical formulations, plant raw materials, etc. To solve the problem, to achieve the effect of fast and economical extraction, separation and purification, efficient and fast extraction process, and perfect extraction and preparation process

Active Publication Date: 2017-08-18
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the increasing scale of production of Chinese patent medicines, the natural reproduction rate of single-sided needles is low and the growth is relatively slow, resulting in an increasingly scarce natural resource, which is in short supply and gradually threatens the normal production of related pharmaceutical factories.

Method used

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  • Extraction and separation purification method for total alkaloids in zanthoxylum dissitum hemsl
  • Extraction and separation purification method for total alkaloids in zanthoxylum dissitum hemsl
  • Extraction and separation purification method for total alkaloids in zanthoxylum dissitum hemsl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Effect of Different Solvents on Microwave Extraction

[0038] Weigh 10 parts of each 10g of mixed crude drug powder of single-faced needle rhizome after vacuum drying, add 20mL of 1% (v / v) ammonia water to infiltrate respectively, and then distilled water, absolute ethanol, n-butanol, chloroform, acetone, acetic acid Ethyl ester, ethanol-ethyl acetate (v:v=1:1), ethanol-n-butanol (v:v=1:1), ethanol-chloroform (v:v=1:1), ethanol-acetone ( v:v=1:1) is the extraction solvent, the crude drug powder and 10 times the amount of solvent are put into the flask and mixed evenly, after soaking for 15 hours, microwave extraction at 70°C for 20 minutes, the extract is concentrated to obtain the extract, and the water-soluble alkaloids are calculated The results are shown in Table 1. So choose ethanol-acetone (1:1) mixed solution as the extractant.

[0039] Table 1 The effect of microwave extraction of single-sided needles with different solvents

[0040]

Embodiment 2

[0042] Effects of Rhizome Powders with Different Particle Sizes and Mixing Ratio on Microwave Extraction

[0043] Weigh 5 parts of dried A. chinensis root and stem powder, pass through 40, 60, 80, and 100 mesh sieves respectively, and mix them according to the ratio of 1:1, 1:2, and 1:3 to form a 5g mixed sample. Add 50mL of 80% mixed extraction solvent respectively, the ratio of solid to liquid is 20g / mL, soak for 15h, then extract by microwave at 70°C for 20min, filter, repeat the extraction once with the filter residue, combine the two filtrates, and calculate the total alkaloid extraction amount, the result As shown in Table 2, different particle sizes and different rhizome mixing ratios will also affect the extraction effect of the total alkaloids of T.

[0044] Table 2 The effect of different mixing ratios of rhizomes and the number of sieves on the extraction of single-sided needles

[0045] Rhizome mixing ratio 1:1 Rhizome mixing ratio 1:2 Rhizome mixi...

Embodiment 3

[0048] Effect of extraction solvent concentration on extraction effect

[0049] Take by weighing 5 parts of dry rhizome powder of Shamrock, each 5g. Add 50mL of mixed extraction solvents of 50%, 60%, 70%, 80%, and 90% respectively, and the ratio of solid to liquid is 20g / mL. After soaking for 15h, extract by microwave at 70°C for 20min, filter, and use the same method to repeatedly extract the filter residue once, and combine Two filtrates were used to calculate the total alkaloid extraction. In the range of 50% to 90% of the fraction of the mixed extraction liquid, with the increase of the fraction of the mixed extraction liquid, the extraction rate of the total alkaloids gradually increased. When the mixed extraction liquid fraction was greater than 80%, the total alkaloid extraction rate decreased ( figure 1 ). Therefore, when the fraction of the mixed liquid is 80%, the polarity of the extracting liquid is the closest to that of the total alkaloids of T. chinensis, and ...

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Abstract

The invention discloses an extraction and separation purification method for total alkaloids in zanthoxylum dissitum hemsl. The extraction and separation purification method has the advantages that extraction and separation purification of the total alkaloids in the zanthoxylum dissitum hemsl are optimized by the aid of response processes, the extraction and separation purification method is speedy and economical, extraction and preparation technologies for the total alkaloids in the zanthoxylum dissitum hemsl can be improved and are efficient and speedy as compared with reported extraction technologies, and products are high in purity.

Description

technical field [0001] The invention relates to an extraction and preparation method of extracting total alkaloids of S. chinensis by a response surface method, and using a macroporous resin to purify and separate the product, and belongs to the technical field of plant active ingredient extraction. Background technique [0002] Zanthoxylum Dissitum Hemsl, also known as shell pepper. Roots, stems and leaves can be used as medicine. It has clinical effects of dispelling blood stasis, relieving pain, detoxifying and reducing swelling. Medicinal ingredients, among which the currently known alkaloids contained in single-sided needles include white mossine, two-faced needles, zanthoxylin, genusine, sanguinarine, and zanthoxylum alkaloids, which are effective for inflammation and inhibition of tumors. effective. In recent years, due to the increasing scale of production of Chinese patent medicines, the natural reproduction rate of single-sided needles is low and the growth is re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/758A61P31/04
CPCA61K36/758A61K2236/333A61K2236/55
Inventor 王平周韬孙吉康李猛戴明龙
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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