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Preparation method and application of complex of alkaline protease and polydimethylsiloxane

A technology of polydimethylsiloxane and protease, which is applied in the field of compound preparation of alkaline protease and polydimethylsiloxane, and can solve problems such as inability to industrialize

Active Publication Date: 2020-01-17
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention aims at the problem that the prior art cannot be industrialized, and provides a method for preparing a compound of alkaline protease and polydimethylsiloxane and its application

Method used

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  • Preparation method and application of complex of alkaline protease and polydimethylsiloxane
  • Preparation method and application of complex of alkaline protease and polydimethylsiloxane

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preparation example Construction

[0035] A preparation method of a compound of alkaline protease and polydimethylsiloxane, which comprises the following steps:

[0036] 1. Stir and mix the alkaline protease, polydimethylsiloxane and curing agent to obtain a mixed liquid;

[0037] 2. Then vacuum until the vacuum degree reaches below 0.120mBar and continue stirring to remove the air bubbles in the mixed liquid;

[0038] 3. Apply the mixed solution after removing air bubbles evenly on the surface of the glass slide, and put it in an environment with a temperature of 16-20°C and a humidity of 60-80% to solidify, so as to obtain an alkaline protease with a thickness of 0.5-1.0mm Complex with polydimethylsiloxane.

[0039] The preparation method of the complex of alkaline protease and polydimethylsiloxane, the stirring speed in the step 1 is 80-120rpm, preferably 100rpm, the stirring temperature is 20-25°C, and the stirring speed in the step 2 is 80- 120rpm, preferably 100rpm, the stirring time is 30-60min, preferab...

Embodiment 1

[0042] A compound of alkaline protease and polydimethylsiloxane, the alkaline protease is embedded in the silicone elastomer, the silicone elastomer is a mixture of the main agent and the curing agent with a weight ratio of 10:1, the main The agent is polydimethylsiloxane, the curing agent is silicone oil, and the alkaline protease comprises 40% water, 50% glycerin and 10% subtilisin by weight, and the weight of alkaline protease to the silica gel elastomer is The ratio is 1:10.

[0043] A preparation method of a compound of alkaline protease and polydimethylsiloxane, which comprises the following steps:

[0044] 1. Stir and mix the alkaline protease, polydimethylsiloxane and curing agent to obtain a mixed liquid;

[0045] 2. Then vacuum until the vacuum degree reaches below 0.120mBar and continue stirring to remove the air bubbles in the mixed liquid;

[0046] 3. Spread the mixed solution after removing air bubbles evenly on the surface of the glass slide, and place it in a...

Embodiment 2

[0050] Get 10g alkaline protease (i.e. Alcalase enzyme) (consist of 41% water, 50% glycerol and 9% subtilisin, Novozymes, Denmark), 90.9g main agent (polydimethylsiloxane, purchased from Dow Corning Company) and 9.1 g curing agent (silicone oil, purchased from Dow Corning Company) were stirred and mixed at 25° C. and 100 rpm. Then evacuate to 0.12mBar and continue to stir for 50min. Then, the mixed liquid after the removal of air bubbles was coated on the surface of a glass slide with a specification of 38 mm × 26 mm by manual coating, and placed in an incubator with a temperature of 18 ° C and a humidity of 65% for 24 hours to obtain alkaline protease and polymer. Composite material of dimethylsiloxane.

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Abstract

The invention discloses alkaline proteinase and polydimethylsiloxane composite and a preparation method and application thereof; the alkaline proteinase is embedded in silicone elastomer, the silicone elastomer is a mixture of a main agent and a curing agent in a weight ratio of (8-12):1, the main agent is polydimethylsiloxane, the curing agent is silicone oil, the alkaline proteinase comprises, by weight 35-45% of water, 50% of glycerol and 5-15% of subtilin, the weight ratio of the alkaline proteinase and the silicone elastomer is 1:(5-15), and the composite acts as an inhibitor for formation of biofilm of marine microorganisms. The alkaline proteinase is embedded in the polydimethylsiloxane having good chemical inertness and zero toxicity, and the proteinase and polydimethylsiloxane composite resistant to the formation of biofilm of marine microorganisms is formed. The composite has a wide range of service temperature and pH value and is suitable for actual use.

Description

technical field [0001] The invention relates to a preparation method and application of a compound of alkaline protease and polydimethylsiloxane, and belongs to the technical field of preparation of marine antifouling materials. Background technique [0002] Generally speaking, once an attachment substrate such as a glass slide with a clean surface is immersed in the ocean, within a few minutes to several hours, the surface of the attachment substrate will rapidly form a microbial film. Marine attached bacteria and benthic diatoms are important components of microbial envelopes. Marine microbial film exists on the underwater surface of ships, culture cages and cooling water systems of power plants, which accelerates the corrosion of metal materials and the degradation of non-metal materials, and affects the subsequent attachment of large fouling organisms such as mussels and barnacles. Thereby causing serious consequences and easily causing huge economic losses. Since 2008...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L83/04C08K5/053C08K5/00
CPCC08L83/04C08L2205/025C08K5/053C08K5/0058
Inventor 杨金龙郭行磐梁箫李家乐
Owner SHANGHAI OCEAN UNIV