Unlock instant, AI-driven research and patent intelligence for your innovation.

Method and system for separating plasma proteins from blood products using diatomaceous earth

A technology for separating plasma and blood products, which is applied in the field of biopharmaceuticals, can solve problems such as residue, time-consuming, and secondary pollution, and achieve the effects of protecting structure and activity, avoiding product contamination, and accurately controlling

Active Publication Date: 2021-06-25
WUHAN ZHONGYUAN RUIDE BIOLOGICAL PROD CO LTD
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned device is easy to generate dust during the diatomite addition process, and the device itself cannot be thoroughly cleaned and disinfected, resulting in secondary pollution, and there is a great potential pollution; diatomite is in the form of solid powder, and the feeding car is vacuum suction , there are residues, so the addition amount cannot be accurately controlled, and the operation is cumbersome, time-consuming and inefficient
[0004] Thus, the method of adding diatomaceous earth in the process of separating plasma protein from blood products needs to be improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and system for separating plasma proteins from blood products using diatomaceous earth
  • Method and system for separating plasma proteins from blood products using diatomaceous earth
  • Method and system for separating plasma proteins from blood products using diatomaceous earth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Select three batches of human plasma proteins, the batch numbers are 20160610, 20160611, and 20160612 respectively, and use the method of the embodiment of the present invention to process the above three batches of samples, the steps are as follows:

[0076](1) After the raw plasma is collected by breaking the bag, it is centrifuged under the condition of 0-4 degrees. After the centrifuged plasma is made into components I+II+III, according to the weight of the components I+II+III obtained after production, Calculate the amount of diatomaceous earth according to 20g / Kg component I+II+III product (W / W).

[0077] (2) According to the amount of diatomaceous earth, prepare diatomite qualified for dry baking, and dry bake at 250° C. for 1 hour. Accurately weigh the dry-baked diatomite, prepare it according to the ratio of diatomite: component I+II+III balance solution (1:3.5), dissolve the diatomite in the liquid mixing tank to obtain homogeneous diatomite liquid, start the...

Embodiment 2

[0081] Collect the three batches of component I+II+III reaction liquids of the corresponding batches of Example 1, and use the method of the embodiment of the present invention to process the above-mentioned three batches of samples respectively, the steps are as follows:

[0082] (1) After the reaction solution of Example 1 is finished making component IV, the amount of diatomaceous earth is calculated according to 15 g / kg component IV product (W / W) according to the weight of the component IV product obtained after making.

[0083] (2) According to the amount of diatomite used, prepare diatomite qualified for dry roasting, and dry roast at 230° C. for 1 hour. Accurately weigh the dry-baked diatomite, prepare it according to the ratio of diatomite:component IV equilibrium solution (1:4), dissolve the diatomite in the liquid mixing tank to obtain a diatomite homogeneous solution, and start the homogeneous solution. Mass liquid delivery procedure, adding it to the reaction tank ...

Embodiment 3

[0087] Collect three batches of component IV reaction liquids of the corresponding batches of Example 2, and use the method of the embodiment of the present invention to process the above three batches of samples respectively, the steps are as follows:

[0088] (1) Completing the preparation of component V, separation by pressure filtration, and obtaining the precipitation of component V. Dissolve the obtained component V precipitate with -1-0°C 4% ethanol solution, the ratio of component V precipitate: 4% ethanol solution is 1:3. The component V solution is obtained, and the pH value of the component V solution is adjusted to a range of 4.0 to 4.6 to obtain a component V product. Calculate the amount of diatomaceous earth according to the weight of the component V product obtained by obtaining the component V product (W / W) at 2.5 g / kg.

[0089] (2) According to the amount of diatomite used, prepare diatomite qualified for dry baking, and dry bake at 200° C. for 1 hour. Accu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
densityaaaaaaaaaa
specific surface areaaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method and a system for separating plasma protein from blood products by using diatomite, wherein the method for separating plasma protein from blood products by using diatomite includes: first stirring the diatomite and the balance liquid, so that Obtaining a homogeneous solution; transporting the homogeneous solution through a delivery pipeline and performing second stirring with a blood product containing plasma protein to obtain a mixed solution; and performing pressure filtration on the mixed solution to obtain the plasma protein. In this method, the original diatomite solid-state addition mode is changed to a liquid mode addition, so as to avoid dust generation during the diatomite addition process, and the homogeneous solution containing diatomite is added through the conveying pipeline, realizing the diatomite closed type The addition process does not produce dust or residue, and can accurately control the amount of diatomaceous earth added, and avoid product pollution.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a method for separating plasma proteins from blood products using diatomite and a system for separating plasma proteins from blood products. Background technique [0002] Diatomite is a biogenic siliceous sedimentary rock mainly composed of diatom remains. Diatomite is often accompanied by biological remains, and various clay minerals (kaolinite hydromica, etc.) and detrital minerals ( quartz, feldspar, etc.). In the production process of blood products, diatomaceous earth is used as a filter aid, and the solid impurity particles suspended in the liquid are trapped on the surface of the medium and in the channel through sieving, depth effect, and adsorption, so as to achieve the purpose of solid-liquid separation. . Diatomaceous earth is in the form of light powder, which is easy to generate dust when used. [0003] In the prior art, there are two ways to add diatomaceous ear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K14/745C07K14/755C07K14/765C07K1/34C07K1/02
CPCC07K14/4717C07K14/745C07K14/755C07K14/765
Inventor 苏元谢松平王珍
Owner WUHAN ZHONGYUAN RUIDE BIOLOGICAL PROD CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com