Method for lowering seed shattering of hybrid progeny of cultivated rice and weedy rice by using SH4 gene silencing

Abstract

The invention belongs to the technical field of bioenvironmental safety detection, and particularly relates to a method for lowering seed shattering of a hybrid progeny of cultivated rice and weedy rice by using a SH4 gene silencing. According to the method, a gene silencing technology is applied to make the function of the seed shattering gene SH4 of the cultivated rice loss, once cultivated rice transgenes which contain SH4 gene silencing elements are transferred to a weedy rice colony through gene flow, the risk of transgene diffusion can be lowered since the seed shattering is lowered; meanwhile, the seed shattering and diffusibility of the weedy rice colony which contains the SH4 gene silencing elements can be lowered greatly, accordingly through the gene flow from the cultivated rice to the weedy rice, characteristics of seed shattering lowering can be transferred to the weedy rice colony, and the purposes of controlling the diffusion of the weedy rice colony and lowering the risk of the weedy rice are achieved. The method can greatly lower the environmental safety risk which can be caused after the transgenes escape to the weedy rice, lower the weedy rice diffusion and endanger and avoid economic loss, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological environment safety detection, and in particular relates to a method for reducing seed shattering of hybrid offspring of cultivated rice and weedy rice. Background technique [0002] While the commercial application of transgenic crops brings huge economic benefits, there are also some potential safety issues of genetically modified organisms: some transgenes (target genes) with natural selection advantages, such as: disease resistance, insect resistance and herbicide resistance transgenes, Escape from cultivated crops to their wild relatives can occur through gene flow, resulting in difficult-to-control weeds and potential environmental biosecurity concerns. Therefore, in order to avoid or reduce the harm that may be caused by the escape of transgenes, transgene attenuation technology can be used, that is, the expression of genes that are disadvantageous to the wild relatives of cultivated crop...

AI Technical Summary

Problems solved by technology

[0002] While the commercial application of genetically modified crops brings huge economic benefits, there are also some potential safety issues of genetically modified organisms: some transgenes (target genes) with natural selection advantages, such as: disease-resistant, insect-resistant and herbicide-resistant transgenes, Can escape from cultivated crops to their wild relatives through gene flow, creating difficult-to-control weeds with potential environmental biosecurity concerns

Weedy rice has a strong seed shattering property. Mature rice seeds are easy to fall into the soil of the paddy field due to a little natural wind or external mechanical force, and cannot be harvested, resulting in a huge loss of grain production.

However, the seed shattering of cultivated rice is not strong

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