use sh4 Method for gene silencing to reduce seed shattering of hybrid offspring of cultivated rice and weedy rice

Abstract

The invention belongs to the technical field of bio-environment safety detection, and specifically relates to a SH4 A method for gene silencing to reduce seed shattering of hybrid offspring of cultivated rice and weedy rice. The present invention makes the grain shattering gene ( SH4 ) loss of function, including SH4 Once the cultivated rice transgene with the gene silencing element is transferred to the weedy rice population through gene drift, the risk of transgene spread will be reduced due to the decreased seed shattering; SH4 The seed shattering and dispersal ability of the weedy rice population with the gene silencing element will be greatly reduced, so that the trait of reduced seed shattering can be transferred to the weedy rice population through the gene drift from cultivated rice to weedy rice to achieve control The purpose of weedy rice colony spreading and reducing the harm of weedy rice. The invention can greatly reduce the environmental safety risk that may be caused after the transgene escapes into the weedy rice, reduces the spread and damage of the weedy rice, avoids economic loss, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological environment safety detection, and in particular relates to a method for reducing seed shattering of hybrid offspring of cultivated rice and weedy rice. Background technique [0002] While the commercial application of transgenic crops brings huge economic benefits, there are also some potential safety issues of genetically modified organisms: some transgenes (target genes) with natural selection advantages, such as: disease resistance, insect resistance and herbicide resistance transgenes, Escape from cultivated crops to their wild relatives can occur through gene flow, resulting in difficult-to-control weeds and potential environmental biosecurity concerns. Therefore, in order to avoid or reduce the harm that may be caused by the escape of transgenes, transgene attenuation technology can be used, that is, the expression of genes that are disadvantageous to the wild relatives of cultivated crop...

AI Technical Summary

Problems solved by technology

[0002] While the commercial application of genetically modified crops brings huge economic benefits, there are also some potential safety issues of genetically modified organisms: some transgenes (target genes) with natural selection advantages, such as: disease-resistant, insect-resistant and herbicide-resistant transgenes, Can escape from cultivated crops to their wild relatives through gene flow, creating difficult-to-control weeds with potential environmental biosecurity concerns

Weedy rice has a strong seed shattering property. Mature rice seeds are easy to fall into the soil of the paddy field due to a little natural wind or external mechanical force, and cannot be harvested, resulting in a huge loss of grain production.

However, the seed shattering of cultivated rice is not strong

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