Tissue culture method of tissue culture seedling of moringa oleifera
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A technology for tissue culture and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of yellowing and easy breaking of seedlings, and achieve the goal of improving the degree of lignification, shortening the cycle, and saving costs Effect
Inactive Publication Date: 2017-08-29
上海杉一植物科技有限公司
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[0017] This experiment aims at the subculture process of a kind of Moringa oleifera, and solves the probl
Method used
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Embodiment 1
[0036] A tissue culture rapid propagation method of Moringa, comprising the following steps:
[0037] (1) Experimental material must be selected: the Moringa oleifera tissue culture seedlings whose growth is neat, plant height is 3.5-4.5cm, subculture cycle is 21-25 days, leaf color is green or tender green are selected as culture material;
[0038] (2) Specific experimental steps:
[0039] (2.1) Cutting of the material: remove the leaves, keep the top buds and heart leaves, leave the petiole 0.1-0.2 cm, cut into 0.5-0.8 cm stem segments, each segment with 1-2 axillary buds;
[0040] (2.2) subculture of Moringa oleifera; terminal buds and stem sections are inoculated separately, and evenly inoculated into No. 8 subculture medium (LM-8);
[0041] Among them, the improved MS (KNO 3 0.95-1.43g / L, NH 4 NO 3 0.81-1.24g / L, MgSO 4 ·7H 2 O 0.19-0.28g / L, KH 2 PO 4 0.09-0.13g / L, CaCl 2 2H 2 O 0.22-0.44g / L, Na 2 EDTA·2H 2 O0.002-0.04g / L, FeSO 4 ·7H 2 O 0.015-0.03g / L, MnSO ...
Embodiment 2
[0048] A tissue culture rapid propagation method of Moringa, comprising the following steps:
[0049] (1) Experimental material must be selected: the Moringa oleifera tissue culture seedlings whose growth is neat, plant height is 3.5-4.5cm, subculture cycle is 21-25 days, leaf color is green or tender green are selected as culture material;
[0050] (2) Specific experimental steps:
[0051] (2.1) Cutting of the material: remove the leaves, keep the top buds and heart leaves, leave the petiole 0.1-0.2 cm, cut into 0.5-0.8 cm stem segments, each segment with 1-2 axillary buds;
[0052] (2.2) subculture of Moringa oleifera; terminal buds and stem sections are inoculated separately, and evenly inoculated into No. 8 subculture medium (LM-8);
[0053] Among them, step (2.2) improved MS (KNO 3 0.95-1.43g / L, NH 4 NO 3 0.81-1.24g / L, MgSO 4 ·7H 2 O 0.19-0.28g / L, KH 2 PO 4 0.09-0.13g / L, CaCl 2 2H 2 O 0.22-0.44g / L, Na 2 EDTA·2H 2 O 0.002-0.04g / L, FeSO 4 ·7H 2 O 0.015-0.03g...
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Abstract
The invention relates to a tissue culture method of a tissue culture seedling of moringa oleifera. The tissue culture method comprises the following steps of (1) selecting experiment materials; (2) specific experiment steps: (2.1) obtaining of a sterile explant: cutting leaves, remaining a terminal bud and interior leaves, remaining a 0.1 to 0.2cm petiole, cutting into 0.5 to 0.8cm stem sections, and remaining 1 to 2 axillarybuds on each section; (2.3) subculture of the moringa oleifera; (2.4) culturing for 21 to 25 days, wherein the height of the longitudinal growth plant is 4.5cm or higher, the side buds of the plant respectively germinate, and the statistic average proliferation ratio reaches 1:6; (2.5) culturing for 18 days in an LM-8 culture medium, wherein the average height of the plant reaches 4cm, the rooting rate reaches 98%, the root length is greater than 2cm, and the number of roots is greater than 3; (2.6) domesticating of the tissue culture seedling: transplanting the rooting tissue culture seedling of the moringa oleifera into a greenhouse, unscrewing a bottle cap, exercising the seedling for 2 to 3 days, and transplanting to a sand bed, wherein the survival rate reaches 95% or above. The tissue culture method has the advantages that the problems of yellowing, crispness and easiness in breaking in the tissue culture process are solved, the expanding propagation and rooting are completed in the bottle at one step, and the cost is reduced by 40% or more.
Description
technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method of Moringa oleifera tissue culture seedlings, and belongs to the field of biotechnology. Background technique [0002] Moringa (Moringa oleifera Lam.), also known as drumstick tree, milk tree, Chinese toon tree, horse radish, and bean, is a deciduous woody vegetable and oil plant of the genus Moringaceae (Moringaceae) The plant is named Moringa because of its spicy root. Moringa is native to the southern foothills of the Himalayas in northern India, and is now widely planted in more than 30 tropical and subtropical countries and regions in Asia, Africa and America. [0003] The whole body of Moringa is a treasure, and its roots, stems, leaves, flowers, fruits, seeds, etc. can be used, and are rich in nutrients. It is widely used in various fields such as agriculture, industry, animal husbandry, medicine, and beauty. A rare dish integrating delicacy, nutrition a...
Claims
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