Aegiceras corniculatum leaf n-butanol extract and its preparation method and use in treatment on colon cancer

A technology of n-butanol and extract, which is applied in the application field of tung flower leaf n-butanol extract and its preparation and treatment of colon cancer, which can solve the problems of tumor variation, drug resistance, and large toxic and side effects

Active Publication Date: 2017-08-29
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

At present, the methods of Western medicine for the treatment of colon cancer mainly include surgery, radiotherapy, chemotherapy and the combined treatment of the three, but the side effects are relatively large, and it is easy to cause tumor mutation and drug resistance.

Method used

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  • Aegiceras corniculatum leaf n-butanol extract and its preparation method and use in treatment on colon cancer
  • Aegiceras corniculatum leaf n-butanol extract and its preparation method and use in treatment on colon cancer
  • Aegiceras corniculatum leaf n-butanol extract and its preparation method and use in treatment on colon cancer

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Embodiment Construction

[0022] One, the preparation of tung flower leaf n-butanol part extract (NACL)

[0023] 1. Medicinal materials: Tung flower leaves are collected in Fangchenggang, Guangxi, and identified by Xu Mingben, Guangxi Beibu Gulf Marine Research Center as the leaves of the Tung flower tree of the family Taurus.

[0024] 2. Extraction and separation: Dried the leaves of tung flower and beat them into coarse powder (2500g), added 25 times the amount (62.5L) of 95% ethanol and soaked them for 7 days; Add pure water to the total extract to dissolve into a suspension, volatilize until there is no ethanol smell, add petroleum ether for extraction, obtain the lower aqueous phase, add ethyl acetate to the lower aqueous phase to continue extraction, and continue to add n-butyl acetate to the obtained lower aqueous phase Continue the extraction with alcohol to obtain the extract of the n-butanol part of the upper layer, and dry the extract of the n-butanol part of the upper layer in a water bath ...

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Abstract

The invention provides aegiceras corniculatum leaf n-butanol extract and a preparation method thereof. The preparation method comprises adding aegiceras corniculatum leaves into ethanol, soaking the aegiceras corniculatum leaves to obtain ethanol total extract of aegiceras corniculatum leaves, volatilizing the ethanol in the ethanol total extract, adding petroleum ether into the total extract, carrying out extraction, collecting a water phase in the lower layer, adding ethyl acetate into the water phase in the lower layer, carrying out extraction, collecting a water phase in the lower layer, adding n-butanol into the water phase in the lower layer, carrying out extraction, collecting n-butanol extract in the upper layer, carrying out water bath treatment, and carrying out drying. An in-vitro molecular biology experiment result shows that the n-butanol extract of the aegiceras corniculatum leaves can inhibit proliferation of human colon cancer cells HT-29, SW480 and DLD-1, significantly inhibit cloning of human colon cancer cells HT-29, SW480 and DLD-1 and induce apoptosis of cancer cells through adjusting associated proteins in multiple apoptosis signal paths. Therefore, the n-butanol extract of the aegiceras corniculatum leaves has a potential application prospect in preparation of drugs for treating colon cancer and can be used for preparation of an anti-colon cancer drug.

Description

technical field [0001] The invention belongs to the technical field of tung flower, in particular to a n-butanol extract of tung flower leaves and its preparation and application for treating colon cancer. Background technique [0002] Tung flower tree (Aegiceras corniculatum), also known as black olive, red gourd, black foot stem, etc., is a plant of the genus Aegiceras and is a common mangrove plant. It is mainly distributed in Guangxi, Guangdong, Fujian and Hainan Islands in my country. , India, Indochina, the Philippines and Australia are also distributed. Tung flower tree is a traditional Chinese medicine. Its bark and leaves are often boiled to treat diseases such as asthma, diabetes and rheumatism. Modern pharmacological research has found that the extract of tung flower tree has pharmacological effects such as cytotoxicity, antifungal, antidiarrheal, antioxidation and liver protection. Although it has been reported that the derivatives obtained from the petroleum et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61K127/00
CPCA61K36/185A61K2236/333A61K2236/51A61K2236/55
Inventor 罗花邓家刚侯小涛郝二伟杜正彩易湘西谢滟谭德超冯小慧
Owner GUANGXI UNIV OF CHINESE MEDICINE
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