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A generation of calyx polysaccharide and its preparation method and application

A technology of calyx and polysaccharides, which is applied in the fields of allergic diseases, antineoplastic drugs, antiviral agents, etc.

Active Publication Date: 2019-08-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are almost no research reports on Daidaihua polysaccharides.

Method used

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  • A generation of calyx polysaccharide and its preparation method and application
  • A generation of calyx polysaccharide and its preparation method and application
  • A generation of calyx polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Prepare the calyx crude polysaccharides (CAVAPs) from the calyx of the generation:

[0066] (1) Pass through a 20-mesh sieve after the generation calyx is crushed, weigh 100g, and heat and reflux extraction with distilled water, wherein the ratio of water to material is 20:1 (mass ratio), the reflux extraction temperature is 75°C, and the reflux extraction time is 4h. The number of reflux extractions was 3 times; then the extracts were combined, centrifuged at 4000r / min for 10min, the supernatant liquid was taken, filtered through filter paper for 3 times, and the filtrate was concentrated to 200mL under reduced pressure at 40°C with a rotary evaporator to obtain a polysaccharide solution;

[0067] (2) D354FD resin was first soaked in distilled water for 12 hours, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 0.5 hours respectively, filtered through 200 mesh gauze, and washed with distilled water until neutral. Obtain pretre...

Embodiment 2

[0070] Example 2 Prepare the calyx crude polysaccharides (CAVAPs) from the calyx of the generation:

[0071] (1) Pass through a 20-mesh sieve after crushing the calyx from generation to generation, weigh 100g, and then heat and reflux extraction with distilled water. The number of extractions was 2 times; then the extracts were combined, centrifuged at 3000r / min for 8min, the supernatant liquid was taken, filtered twice with filter paper, and the filtrate was concentrated to 200mL under reduced pressure at 50°C with a rotary evaporator to obtain a polysaccharide solution;

[0072] (2) D354FD resin was first soaked in distilled water for 24h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 2h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin;

[0073] (3) Mix the polysaccharide solution prepared in step (1) with 200 mL of pretreated D354FD resin, place in a constant tem...

Embodiment 3

[0075] Example 3 Prepare the calyx crude polysaccharides (CAVAPs) from the calyx of the generation:

[0076] (1) Pass through a 20-mesh sieve after the generation calyx is crushed, weigh 100g, and heat and reflux extraction with distilled water, wherein the ratio of water to material is 20:1 (mass ratio), the reflux extraction temperature is 95°C, and the reflux extraction time is 2h. Reflux and extract 4 times, then combine the extracts, centrifuge at 5000 / min for 12 minutes, take the supernatant liquid, filter it 4 times with filter paper, and concentrate the filtrate to 200 mL under reduced pressure at 60°C with a rotary evaporator to obtain a polysaccharide solution;

[0077] (2) D354FD resin was first soaked in distilled water for 18h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 3h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin;

[0078] (3) Mix the polys...

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PUM

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Abstract

The invention belongs to the field of traditional Chinese medicine, and discloses bitter orange calyx polysaccharide and a preparation method and application thereof. The bitter orange calyx polysaccharide is extracted from calyxes of bitter oranges through the steps of hot water reflux extraction, decoloration processing, alcohol precipitation and the like. Refined polysaccharide is obtained by further purifying the rough bitter orange calyx polysaccharide. The rough polysaccharide and the refined polysaccharide have oxidation resistance, tumor resistance and immune enhancement activity, the use dosage is low, and the toxic and side effect is also low under the same dosage.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a calyx polysaccharide from generation to generation and its preparation method and application. Background technique [0002] Polysaccharides are an important class of biopolymers other than proteins and nucleic acids. Modern pharmacological studies have shown that polysaccharides have a variety of physiological activities, including anti-oxidation, anti-tumor, anti-virus, lowering blood lipids, delaying aging, and enhancing immune function. Immunomodulatory effect is the most important bioactive function of polysaccharides, which has always been a research hotspot. The adverse reactions and side effects of immune bacterial polysaccharides and synthetic compounds have attracted widespread attention. However, most polysaccharides derived from higher plants are non-adverse substances and will not cause major side effects on the body. Therefore, p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04A61P35/00A61P39/06
Inventor 姜建国申春燕宋德幸
Owner SOUTH CHINA UNIV OF TECH
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