Compound, muscarinic m receptor antagonist, composition and application

A receptor antagonist and compound technology, which is applied in the discovery and application field of the action mechanism of the active ingredients of natural medicines, to achieve the effect of broadening the scope of clinical application, small side effects, and good effect.

Active Publication Date: 2019-10-11
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because atropine has effects on glands, smooth muscle, and nervous system, it has multiple side effects while treating diseases. In contrast, the discovery of M receptor antagonists with different structures, especially new compounds themselves, may have fewer side effects

Method used

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  • Compound, muscarinic m receptor antagonist, composition and application
  • Compound, muscarinic m receptor antagonist, composition and application
  • Compound, muscarinic m receptor antagonist, composition and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Purification and Preparation of Alkaloid Compounds in Anisodamine Tangut

[0039] 1. Experimental instruments and reagents

[0040] Tangut Anisopolamine medicinal material was collected in Qinghai by the Northwest Plateau Botanical Institute, and was identified by Teacher Mei Lijuan. Column XCharge C18 (4.6×250mm, 7μm, Huapu Company), XCharge C18 (100×280mm, 7μm, Huapu Company), XCharge SCX (100×316mm, 7μm, Huapu Company), XCharge C18 (20×250mm , 7 μm, Huapu Company), anhydrous sodium sulfate, sodium dihydrogen phosphate dihydrate were purchased from Sinopharm Group, ethanolamine, phosphoric acid, and formic acid were purchased from Bailingwei, and preparative chromatography grade acetonitrile was purchased from Anhui Shilian.

[0041] 2. Purification and preparation of compounds

[0042] Extraction is divided into two batches, each batch is 25kg, soaked in 80L ethanol, heated to reflux for 2h, and extracted three times respectively to obtain 350L extract. ...

Embodiment 2

[0043] The DMR signal characteristic of embodiment 2 muscarinic M receptor antagonist on Epic platform

[0044] 1. Method

[0045] 1.1 Cell culture

[0046] HT29 human colon cancer cells were obtained from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences (Shanghai, China). HT29 cells were treated with McCoy’s 5A medium (GIBCO, product number 12800017, containing D-Glucose 4500.0mg / L, adding NaHCO 3 2.2g / L), at 37°C, with a volume content of 5% CO 2 (air) incubator.

[0047] 1.2 Cell Viability Experimental Method of Muscarinic M Receptor Antagonists

[0048] HT29 cells were treated with 2×10 4 Individuals / well density seeded to In a 384-well biosensor microplate, placed at 37°C, with a volume content of 5% CO 2 (air) incubator for 22 h, washed with HBSS buffer once before detection, then added 30 μL of HBSS buffer to each well, placed in The system was equilibrated and incubated for 1h. After balancing first in Establish a ...

Embodiment 3

[0053] Example 3 MMPVP has antagonistic activity on muscarinic M receptors

[0054] The dose-effect relationship of MMPVP, a compound with strong M receptor antagonistic activity in activity screening, was investigated using label-free cell target pharmacology techniques. First, 10 μL of the compound to be tested was added to the inoculated HT29 cells 384-well biosensor microplates, in The system was monitored for 60 minutes, and then 10 μL of acetylcholine (16 μM) was added to continue monitoring for 60 minutes. The DMR signal induced by the compound is plotted with the action time, such as image 3 a (the compound concentration is diluted stepwise from 200 μM to 48.8nM, and the corresponding DMR curve has little change); the DMR response signal caused by adding acetylcholine in the second step is plotted with the action time, and the results are as follows image 3 b (The compound concentration was serially diluted from 200 μM to 48.8 nM, and the corresponding DMR signa...

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Abstract

The invention discloses a caffeic acid derivative containing a saturated nitrogen heterocycle isolated from Solanaceae plants, or a pharmaceutically acceptable salt thereof. The compound provided by the invention is a muscarinic M receptor antagonist, which can be used for spasm, Treatment of angina pectoris, biliary colic, renal colic, visceral spasm and other diseases. The present invention adopts the unlabeled cell target pharmacology technology, and the dose-effect relationship study shows that the compound antagonizes the DMR response signal caused by acetylcholine in a dose-dependent manner, indicating that the compound has strong muscarinic M receptor antagonistic activity. Compared with known M receptor antagonists such as atropine and scopolamine, the compounds provided by the present invention have very different structures, and are expected to improve the selectivity for muscle glands and nervous system. Current studies have shown that muscarinic M receptors are associated with convulsions, analgesia, sedation, schizophrenia and other diseases, and thus can provide high-efficiency new ligands with clear targets for related diseases.

Description

technical field [0001] The invention belongs to the field of discovery and application of the action mechanism of active ingredients of natural medicines, and relates to a caffeic acid derivative containing a saturated nitrogen heterocycle, its action mechanism and application in antispasmodic medicines. Background technique [0002] Anisodus tanguticus Anisodus tanguticus is a plant of the genus Anisodora in the family Solanaceae. It is a unique plant in China. It is called "Tangchuan Nabao" in Tibetan. Distributed in Gansu, Tibet, Yunnan, Qinghai and other places in mainland China, it grows in areas with an altitude of 2,800 meters to 4,200 meters. It usually grows on hillsides and grassy slopes. It has not been introduced and cultivated artificially. As a traditional Tibetan medicine, Tibetan medicine uses Anisopolamine tanguta and its seeds as medicine, which has anesthetic and analgesic effects [1]. There are few reports on the chemical constituents of Anisodamine tang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D207/06C07D207/08C07D211/12C07D211/22C07D295/03C07D295/096A61K31/40A61K31/445A61K31/4462A61P25/20A61P25/00A61P25/18A61P25/24A61P25/16A61P39/02A61P21/02
Inventor 梁鑫淼杜娜娜刘艳芳张秀莉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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