Method of cultivation of Cordyceps Militaris of 1-deoxynojirimycin enrichment

A cultivation method, the technology of Cordyceps militaris, which is applied in the field of cultivation of Cordyceps militaris, can solve the problems of large dosage and inconvenient administration, and achieve the effect of reducing dosage and improving medicinal value

Inactive Publication Date: 2017-09-15
山东省蚕业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compatibility of Cordyceps and traditional Chinese medicines such as Adenophora, Chuan Fritillaria, Ophiopogon japonicus, Tangerine Peel, Poria, Ejiao, and Mulberry Leaves can not only stop bleeding and resolve phlegm, but also have anti-tumor, liver protection, and hypoglycemic effects. The use of drugs is often interrupted due to the large dosage and inconvenience of taking. For this reason, improving the utilization rate of Cordyceps militaris and simplifying the compatibility of traditional Chinese medicine are the problems that must be solved first.

Method used

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  • Method of cultivation of Cordyceps Militaris of 1-deoxynojirimycin enrichment

Examples

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Embodiment 1

[0045] A method for cultivating Cordyceps militaris rich in active ingredients of mulberry leaves, the steps are as follows:

[0046] (1) Coarsely crush the dried mulberry leaves to about 0.5cm 2 , according to the ratio of mulberry leaf powder to wheat mass ratio of 3:10, add to the wheat culture medium, after sterilizing at 121°C for 1.5h, take it out, cool down, and prepare the enrichment culture medium;

[0047] The wheat culture medium comprises the following components: 30g of wheat and 40ml of water.

[0048] (2) Put the fruiting body of Cordyceps militaris into 0.1% (mass percentage) mercuric chloride solution for disinfection for 3 minutes, wash it with sterile water, scrape off the surface layer, take the middle part tissue block and cut it into 0.1mm tissue slices, inoculate in solid Cultivate in the dark at 25°C for 7 days in the culture medium; then light-sensitive culture for 4 days, select the hyphae that turns orange and inoculate in the liquid culture medium,...

Embodiment 2

[0051] A method for cultivating Cordyceps militaris rich in active ingredients of mulberry leaves, the steps are as follows:

[0052] (1) Add the mulberry leaf water extract extracted by ultrasonic wave according to the solid-liquid ratio of 1:5 (unit g / L), and add it to the wheat medium, the mass ratio of wheat and mulberry leaf water extract is 3:4, 121°C After sterilizing for 1.5 hours, take it out immediately, and cool it down to prepare enriched culture medium;

[0053] The wheat culture medium comprises the following components: 30g of wheat and 40ml of water.

[0054] (2) Put the fruiting body of Cordyceps militaris into 0.1% (mass percentage) mercuric chloride solution for disinfection for 3 minutes, wash it with sterile water, scrape off the surface layer, take the middle part tissue block and cut it into 0.1mm tissue slices, inoculate in solid Cultivate in the dark at 25°C for 7 days in the culture medium; then light-sensitive culture for 4 days, select the hyphae t...

Embodiment 3

[0057] A method for cultivating Cordyceps militaris rich in active ingredients of mulberry leaves, the steps are as follows:

[0058] (1) After homogenizing the fresh mulberry leaves, add them to the wheat culture medium according to the mass ratio of 1:3, and after sterilizing at 121°C for 1.5 hours, take them out immediately, and cool them down to prepare the enrichment culture medium;

[0059] The wheat culture medium comprises the following components: 30g of wheat and 40ml of water.

[0060] (2) Put the fruiting body of Cordyceps militaris into 0.1% (mass percentage) mercuric chloride solution for disinfection for 3 minutes, wash it with sterile water, scrape off the surface layer, take the middle part tissue block and cut it into 0.1mm tissue slices, inoculate in solid Cultivate in the dark at 25°C for 7 days in the culture medium; then light-sensitive culture for 4 days, select the hyphae that turns orange and inoculate in the liquid culture medium, continue to culture ...

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Abstract

The invention relates to a method of cultivation of Cordyceps Militaris of 1-deoxynojirimycin enrichment. The method of cultivation of Cordyceps Militaris containing rich effective active components of Mulberry leaves comprises the steps that 1, raw materials rich in active Mulberry leaves ingredients are added into a culture medium, sterilization is conducted, and enrichment culture medium is prepared; 2, the fruiting body of Cordyceps Militaris is taken, after the cultivation, Cordyceps fungus liquid is prepared; 3, Cordyceps fungus is inoculated into enrichment culture medium, light cultivating is conducted for 3-5 days; culturing is conducted for 2.5-3.5 days; sprouting management is conducted for 14-26 days; harvest is obtained. According to the method of cultivation of Cordyceps Militaris of 1-deoxynojirimycin enrichment, through the addition of Mulberry leaves or Mulberry leaf extract in traditional wheat culture substrate of Cordyceps, cultivation of Cordyceps Militaris is conducted, Cordyceps Militaris of 1-deoxynojirimycin enrichment with active ingredients from mulberry leaves is obtained; after the testing, the contained 1-deoxynojirimycin proportion meets the requirement of traditional Chinese medicine medication, the amount of drug dose is reduced, and the medicinal value of Cordyceps Militaris is dramatically improved.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps militaris rich in 1-deoxynojirimycin, belonging to the technical field of edible fungus cultivation. Background technique [0002] Cordyceps sinensis, known as the "God Grass", has many functions such as hypnosis and sedation, liver and kidney benefits, tonifying deficiency, anti-cancer, anti-cancer, hemostasis, phlegm, and asthma. Great tonic. And Cordyceps militaris (Cordyceps militaris) is called for short Cordyceps militaris, is to parasitize on the pupal bodies such as Noctuidae, is different from another Cordyceps sinensis, and it is distributed all over the world, is a kind of fungus that is both edible and medicinal. To a certain extent, it is used instead of Cordyceps sinensis. [0003] Due to the scarcity of wild Cordyceps resources, experts and scholars are committed to the research on artificial Cordyceps militaris to meet people's growing consumption needs. Studies have shown t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05F11/00
CPCC05F11/00
Inventor 施新琴郭光李化秀乔鹏顾寅钰梁明芝聂玉霞朱红肖连明
Owner 山东省蚕业研究所
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