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Allium mongolicum tissue culture bacteriostat, Allium mongolicum tissue culture medium and Allium mongolicum tissue culture method

A tissue culture medium and a culture medium technology, applied in the field of plant tissue culture, can solve the problems such as the inability to realize the industrialized seedling raising of Mongolian chives, the complicated operation of the plant tissue culture process, and the harsh requirements of the sterile environment, and achieve good bacteriostatic stability and increase Plant height, less toxic and side effects

Inactive Publication Date: 2017-09-15
NANJING XIAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome the harsh requirements on the aseptic environment in the tissue culture process of Mongolian Chinese chives in the prior art, the high cost of sterilization, the cumbersome operation of the plant tissue culture process, and the inability to realize the industrialized seedling cultivation of Mongolian Chinese chives; thereby providing A Mongolian allium tissue culture antibacterial agent capable of high-efficiency antibacterial, safe and environmentally friendly, and promoting the growth of Mongolian allium

Method used

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  • Allium mongolicum tissue culture bacteriostat, Allium mongolicum tissue culture medium and Allium mongolicum tissue culture method
  • Allium mongolicum tissue culture bacteriostat, Allium mongolicum tissue culture medium and Allium mongolicum tissue culture method
  • Allium mongolicum tissue culture bacteriostat, Allium mongolicum tissue culture medium and Allium mongolicum tissue culture method

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Embodiment 1

[0032] The present embodiment provides a kind of tissue culture method of Mongolian Chinese chives, specifically comprising the following steps:

[0033] 1. Preparation of antibacterial agent of Mongolian leek tissue culture

[0034] (1) Weigh 120g of leek, 25g of xugufeng and 35g of black fern, put them into an oven to dry at a temperature of 45°C, and then pulverize the dried plants to obtain plant dry powder;

[0035] (2) Add 3000ml of distilled water to the powder obtained in step (1), and extract three times under reflux in a water bath at 58° C. for three hours each time, and then combine the extracts obtained by the three extractions;

[0036] (3) vacuum suction filtration is carried out to the extract after merging in step (2), the filtrate after suction filtration is concentrated on the rotary evaporator, stops concentrating when filtrate volume dwindles to 150ml, obtains Mongolian leek tissue culture bacteriostatic agent.

[0037] 2. Preparation of Mongolian leek ti...

Embodiment 2

[0055] The present embodiment provides a kind of tissue culture method of Mongolian Chinese chives, specifically comprising the following steps:

[0056] 1. Preparation of antibacterial agent of Mongolian leek tissue culture

[0057] (1) Weigh 110 g of leek, 20 g of Xugufeng and 50 g of black fern, put them into an oven to dry at a temperature of 45° C., and then pulverize the dried plants to obtain dry plant powder;

[0058] (2) Add 3000ml of distilled water to the powder obtained in step (1), and extract three times under reflux in a water bath at 58° C. for three hours each time, and then combine the extracts obtained by the three extractions;

[0059] (3) vacuum suction filtration is carried out to the extract after merging in step (2), the filtrate after suction filtration is concentrated on the rotary evaporator, stops concentrating when filtrate volume dwindles to 150ml, obtains Mongolian leek tissue culture bacteriostatic agent.

[0060] 2. Preparation of Mongolian le...

Embodiment 3

[0075] The present embodiment provides a kind of tissue culture method of Mongolian Chinese chives, specifically comprising the following steps:

[0076] 1. Preparation of antibacterial agent of Mongolian leek tissue culture

[0077] (1) Weigh 125g of leek, 35g of xugufeng and 20g of black fern, put them into an oven to dry at a temperature of 45°C, and then crush the dried plants to obtain dry plant powder;

[0078] (2) Add 3000ml of distilled water to the powder obtained in step (1), and extract three times under reflux in a water bath at 58° C. for three hours each time, and then combine the extracts obtained by the three extractions;

[0079](3) vacuum suction filtration is carried out to the extract after merging in step (2), the filtrate after suction filtration is concentrated on the rotary evaporator, stops concentrating when filtrate volume dwindles to 150ml, obtains Mongolian leek tissue culture bacteriostatic agent.

[0080] 2. Preparation of Mongolian leek tissue ...

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Abstract

The invention discloses an Allium mongolicum tissue culture bacteriostat, a preparation method thereof, an Allium mongolicum tissue culture medium, and a tissue culture method using the Allium mongolicum tissue culture medium. The Allium mongolicum tissue culture bacteriostat is extracted from 44-50 parts by weight of Allium bidentatum, 8-14 parts by weight of Herba Aristolochiae Mollissimae and 8-20 parts by weight of Stenoloma chusanum Ching, and is a natural and safe high-efficiency and wide-spectrum bacteriostasis plant extract product. The preparation method of the Allium mongolicum tissue culture bacteriostat can be used to prepare the wide spectrum bacteriostat having high-bacteriostatic activity without introducing organic solvent pollution. The Allium mongolicum tissue culture bacteriostat is added to the Allium mongolicum tissue culture medium, and is helpful for promoting the growth of plants while inhibiting bacteria. The tissue culture method using the Allium mongolicum tissue culture medium realizes the tissue culture in an open environment; and bulbs are selected as an explant to shorten the tissue culture time of the Allium mongolicum in order to realize high-reproduction rate and high-heredity stability rapid culture.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a Mongolian allium tissue culture antibacterial agent, a Mongolian allium tissue culture medium and a Mongolian allium tissue culture method. Background technique [0002] Mongolian leek (Allium mongolicum Regel) belongs to the rhizome group of Allium genus in the family Liliaceae, also known as Allium mongolicum. It is a perennial herbaceous xerophyte, distributed in six provinces (regions) including Shaanxi, Gansu, Ningxia, Inner Mongolia, Xinjiang and Qinghai. Mongolian leek is rich in various nutrients such as trace elements, vitamins and amino acids necessary for humans and animals. It is not only rich in nutrients such as young leaves and flowers, but also has a unique flavor. It can be eaten cold, fried or pickled. It is also a kind of Excellent forage plant, suitable for cattle, sheep, horses and camels, and can increase fat. In addition, Mongoli...

Claims

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Application Information

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IPC IPC(8): A01N65/42A01P1/00A01H4/00
CPCA01N65/00A01H4/001A01H4/008A01N65/42A01N2300/00
Inventor 王立科陈全战杨平张边江唐宁
Owner NANJING XIAOZHUANG UNIV
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