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Method for promoting momordica grosvenori UGT 4 gene expression

A technology of gene expression and Luo Han Guo, which is applied in the field of plant biology, can solve the problems of promoting the expression of Luo Han Guo UGT4 gene, and achieve the effect of promoting the growth, uniform distribution and biosynthesis of Luo Han Guo

Inactive Publication Date: 2017-09-22
李华政
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on promoting the expression of Luo Han Guo UGT4 gene in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for promoting the expression of Luo Han Guo UGT4 gene, comprising the following steps:

[0030] Step 1, placing the Luo Han Guo tissue-cultured seedlings in the first medium with a methyl jasmonate concentration of 50 μmol / L for cultivation to obtain Luo Han Guo seedlings;

[0031] Step 2: During the process of cultivating the Luo Han Guo seedlings obtained in Step 1, apply an ethanol solution with a methyl jasmonate concentration of 50 μmol / L.

[0032]In the first step, the first culture medium also includes: MS, 1 mg / L 6-BA, 0.2 mg / L IBA, 3 g / L agar, 20 g / L sucrose, and 0.5 g / L activated carbon. Wherein, 6-BA is 6-benzylaminoadenine, and IBA is indole butyric acid.

[0033] The cultivation method of the Luo Han Guo tissue culture seedlings in the step 1 is as follows: the relative humidity is 60%, the light intensity is 1400 lux, the light time is 8h / d, and the temperature is 21°C and cultivated for 30d.

[0034] In the step 2, during the cultivation of Luo...

Embodiment 2

[0036] A method for promoting the expression of Luo Han Guo UGT4 gene, comprising the following steps:

[0037] Step 1, placing the Luo Han Guo tissue-cultured seedlings in the first medium with a methyl jasmonate concentration of 250 μmol / L for cultivation to obtain Luo Han Guo seedlings;

[0038] Step 2: During the process of cultivating the Luo Han Guo seedlings obtained in Step 1, apply an ethanol solution with a methyl jasmonate concentration of 250 μmol / L.

[0039] In the first step, the first culture medium also includes: MS, 1.5 mg / L 6-BA, 0.3 mg / L IBA, 3.5 g / L agar, 30 g / L sucrose, and 1 g / L activated carbon.

[0040] The cultivation method of the Luo Han Guo tissue culture seedlings in the step 1 is as follows: the relative humidity is 63%, the light intensity is 1400 lux, the light time is 8h / d, and the temperature is 23°C and cultivated for 30d.

[0041] In the step 2, during the cultivation of Luo Han Guo, after 25 days after the pollination of Luo Han Guo, the e...

Embodiment 3

[0043] A method for promoting the expression of Luo Han Guo UGT4 gene, comprising the following steps:

[0044] Step 1, placing the Luo Han Guo tissue-cultured seedlings in the first medium with a methyl jasmonate concentration of 400 μmol / L for cultivation to obtain Luo Han Guo seedlings;

[0045] Step 2: During the process of cultivating the Luo Han Guo seedlings obtained in Step 1, apply an ethanol solution with a methyl jasmonate concentration of 400 μmol / L.

[0046] In the first step, the first culture medium also includes: MS, 2mg / L of 6-BA, 0.5mg / L of IBA, 4g / L of agar, 40g / L of sucrose, and 2g / L of activated carbon.

[0047] The cultivation method of the Luo Han Guo tissue culture seedlings in the step 1 is as follows: the relative humidity is 66%, the light intensity is 1400 lux, the light time is 8h / d, and the temperature is 25°C and cultivated for 30d.

[0048] In the step 2, during the cultivation of Luo Han Guo, after 30 days after the pollination of Luo Han Guo,...

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Abstract

The invention provides a method for promoting momordica grosvenori UGT 4 gene expression. The method comprises the following steps that 1, momordica grosvenori tissue culture seedlings are placed into a first culture medium with the jasmonic acid methyl ester concentration being 50-400 micromol / L to be cultured, and momordica grosvenori seedlings are obtained; 2, in the process of obtaining the momordica grosvenori seedlings in the first culture step, an ethanol solution with the jasmonic acid methyl ester concentration being 50-400 micromol / L is applied. A first culture medium and a second culture medium containing jasmonic acid methyl ester are used for culture, the ethanol solution containing jasmonic acid methyl ester is sprayed in the culture process, and UGT4 gene expression in momordica grosvenori can be promoted, so that biosynthesis of mogroside is promoted, and the yield is increased.

Description

technical field [0001] The invention relates to the field of plant biotechnology, and more specifically relates to a method for promoting the expression of the UGT4 gene of Luo Han Guo. Background technique [0002] Mogroside V belongs to the cucurbitane-type tetracyclic triterpenoids. Our research group has deduced the possible biosynthetic pathway of Mogroside V based on the transcriptome data of Luo Han Guo. The precursors for the biosynthesis of mogroside V are isopentenyl diphosphate (IPP) and 3,3-dimethylenylpyrophosphate (DMAPP), both of which are produced by mevalonate (MVA) and methyl Two pathways of erythritol phosphorylation (MEP) are formed, the MVA pathway occurs in the cytoplasm, and the MEP pathway occurs in the plastid. IPP or DMAPP derived from the above two pathways are catalyzed by geranyl pyrophosphate synthase (GPS) to form geranyl pyrophosphate (GPP), and IPP and GPP are catalyzed by farnesyl pyrophosphate synthase (FPS). Then, farnesyl pyrophosphate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01G7/06A01H4/00
CPCC12N5/0025A01G7/06A01H4/00A01H4/001
Inventor 李华政韦荣昌
Owner 李华政
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