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A method for accurately determining the immune dose of antigen prepared by sheep anti-human serum

An antigen immunization and determination method technology, applied in the field of bioengineering, can solve the problems of high price of immunized antigens and low titer of antiserum, and achieve the effects of facilitating accurate operation, reducing antigen immunization dose, and saving production costs.

Active Publication Date: 2021-02-12
海奥斯生物科技镇江有限公司
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AI Technical Summary

Problems solved by technology

[0005] The immunizing antigens used in the current method are expensive and the titer of antiserum is low

Method used

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  • A method for accurately determining the immune dose of antigen prepared by sheep anti-human serum
  • A method for accurately determining the immune dose of antigen prepared by sheep anti-human serum
  • A method for accurately determining the immune dose of antigen prepared by sheep anti-human serum

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Embodiment Construction

[0025] Below in conjunction with example the present invention is described in further detail.

[0026] Antigen dose for the first immunization:

[0027] Benchmark amount: Calculated by 8 parts per billion of sheep weight, such as 50kg sheep, the benchmark amount is 0.4mg.

[0028] The basic amount corresponds to: protein antigen molecular weight 50KD.

[0029] When the molecular weight of the antigen protein is less than 50KD, or greater than 50KD, increase the adjustment coefficient to adjust the dose of antigen immunization.

[0030] When the molecular weight of the antigenic protein is less than 50KD, increase the antigenic amount of the adjustment coefficient, up to 1mg. Wherein, the increment adjustment coefficient=(50-antigen molecular weight KD) / 100. For example, if the molecular weight of an antigen is 20KD and the weight of a sheep is 50kg, then the antigen dose for the first immunization is: reference amount + reference amount * (50-20) / 100.

[0031] When the mo...

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Abstract

The invention discloses a method for accurately determining the antigen immunization dose of sheep anti-human serum, which includes the following steps: (1) Determination of the first immunization antigen dose: reference amount: calculation of 8 / 10000000th of sheep body weight; Quantity correspondence: protein antigen molecular weight 50KD, when the molecular weight of the antigen protein is less than 50KD, or greater than 50KD, increase the adjustment coefficient to adjust the antigen immunization dose; (2) The second immunization dose is twice the first time; (3) The third time The immunization dose is twice that of the second time; (4) From the fourth to the last immunization, the immunization dose is consistent with the third time. The invention has the advantages of: making the amount of immunizing antigen clear in the ambiguous expression in the original textbook; facilitating precise operation; laying the foundation for the industrial production of biotechnology animal antiserum preparation; the price of antigen is expensive, which can minimize the dose of antigen immunization , saving production costs; precise immunization, so that the antiserum titer is higher.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for accurately determining the immune dose of an antigen prepared by goat anti-human serum. Background technique [0002] Antigens and antibodies are two important factors in immunological testing, and are also the basic conditions for the entire immune response. Antigen purification is a prerequisite for the preparation of specific antibodies, and the prepared antibodies can be used to purify and detect antigens. There are two types of antibodies, monoclonal and polyclonal. [0003] Antiserum is serum containing a certain type of antibody molecule with specific immune function, generally animal serum prepared after animals are artificially injected with a certain type of antigen. High titer antisera are used in research work as well as in the diagnosis and treatment of diseases. Whether an antigen can cause antibody production depends on the one hand whether...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/06
CPCC07K16/06C07K2317/20
Inventor 袁金城江晓
Owner 海奥斯生物科技镇江有限公司
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