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Compound medicine and application thereof

A composition and medicine technology are applied in the field of compound pharmaceutical compositions containing conjugated linoleic acid and luteolin to achieve the effects of reducing toxic and side effects, strong anti-proliferative activity, and good prospects for clinical drug development

Inactive Publication Date: 2017-10-20
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combination of luteolin and natural medicine conjugated linoleic acid for the prevention and treatment of malignant tumor lymphoma has not been seen in the relevant literature almost no reports

Method used

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  • Compound medicine and application thereof
  • Compound medicine and application thereof
  • Compound medicine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening of Daudi, Raji and BC-3 cells to optimize the compound pharmaceutical composition of luteolin (LT) and conjugated linoleic acid (CLA).

[0020] Take the cells in the logarithmic growth phase and inoculate 3×10 4 Cells / well were placed on a 96-well plate, and after 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no-drug group and drug-added group, and LT and CLA were used in the drug-added group Group, LT and CLA combined drug groups with different molar ratios, set 4-6 duplicate wells in each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results The inhibition r...

Embodiment 2

[0026] Embodiment 2: Analysis of the combined administration effect of LT and CLA

[0027] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0028] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentra...

Embodiment 3

[0033] Embodiment 3: Luteolin (LT) and conjugated linoleic acid (CLA) compound pharmaceutical composition is made into tablet according to the following prescription:

[0034] The prescription is as follows:

[0035]

[0036] The preparation process is as follows:

[0037] Pass the drug and auxiliary materials through a 80-mesh sieve, fully mix the conjugated linoleic acid with 48 grams of microcrystalline cellulose and 12 grams of carboxymethyl starch sodium, make soft materials with 10% starch slurry, granulate with a 18-mesh sieve, and granulate at 60°C Drying under the hood to obtain granule 1. Fully mix luteolin with 24 grams of microcrystalline cellulose, 15 grams of starch and 8 grams of sodium carboxymethyl starch, make soft material with 10% starch slurry, granulate with a 18-mesh sieve, and dry at 60°C to obtain Granule 2. According to the principle of equal increase, fully mix granule 1 and granule 2, sieve the granules with 16 mesh, add magnesium stearate, mix...

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Abstract

The invention belongs to the field of natural medicines and discloses a compound medicine containing conjugated linoleic acid and luteolin and application thereof. The active ingredient of the compound medicine is composed of linoleic acid and luteolin at the mole ratio of (1:1) to (1:20). The medicine combining with linoleic acid and luteolin has a strong function of cooperatively treating lymphoma.

Description

technical field [0001] The invention belongs to the field of natural medicines, in particular to a compound pharmaceutical composition containing conjugated linoleic acid and luteolin and its application. Background technique [0002] Cancer is a major chronic disease that seriously endangers human health, and has become the second largest killer after cardiovascular disease. Since the beginning of the 21st century, due to the rapid process of modern industrialization, environmental pollution and many other factors, the immune system of lymphocytes has been destroyed and the incidence of lymphoma has been increasing year by year. The drug system is very urgent. [0003] Conjugated linoleic acid (CLA), a natural active substance mainly found in ruminant fat and milk, is believed to be converted into conjugated linoleic acid by microorganisms in the rumen of ruminants. acid. CLA is a general term for a group of positional and geometric isomers of octadecyl-conjugated diene ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/201A61P35/00
CPCA61K31/201A61K31/352A61K2300/00
Inventor 刘连刘腾彭咏波邱桥春黄文柱陈仰新严文
Owner FOSHAN UNIVERSITY
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