Diagnostic system for bone metastasis cancer and operation method thereof

Abstract

The invention discloses a diagnostic system for bone metastasis cancer. The diagnostic system includes a bone puncture system, a bone tissue section preparation system, an immunohistochemistry kit and a protein detection system. The invention further relates to an operation method of the diagnostic system. The method includes the steps of under the guidance of a CT scanner, using a medullo-puncture needle to conduct bone puncture to obtain specimens; conducting decalcification treatment on the obtained specimens by using an EDTA decalcifying solution, and making bone tissue sections; conducting immunohistochemistry preparation by using the immunohistochemistry kit, and conducting standardized detection on different primary tumor sources; conducting prognosis correlation detection by using the protein detection system. The diagnostic system for bone metastasis cancer is optimized overall in process, reagent and device, plenty of specimens are extracted, and the preparation process of the bone tissue sections is shortened; therefore, it is ensured that immunohistochemistry and gene detection can be well conducted on the specimens, and the standardized detection of the different primary tumor sources and prognosis correlation protein detection can be achieved, so that the accuracy of the diagnose is improved.

Description

technical field [0001] The invention belongs to the technical field of biological immunology, and in particular relates to a diagnosis system for bone metastases and an operation method thereof. Background technique [0002] The diagnosis of bone metastases is divided into clinical diagnosis and pathological diagnosis. Pathological diagnosis is the gold standard. Bone tissue needs to be obtained. After making pathological sections, pathological morphology, immunohistochemistry, and gene mutation detection are performed to finally identify bone metastases and guide subsequent treatment. [0003] The deficiencies of commonly used techniques for clinical pathological diagnosis include: [0004] 1) Insufficiency of bone puncture: Bone puncture is the first step in obtaining tissue samples, but as an invasive operation, it is not widely carried out in hospitals at all levels. The amount of tissue samples obtained by bone biopsy is small, and the amount of tissue samples limits ...

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Problems solved by technology

[0004] 1) Insufficiency of bone puncture: bone puncture is the first step in obtaining tissue samples, but as an invasive operation, it is not widely carried out in hospitals at all levels

The amount of tissue samples obtained by bone biopsy is small, and the amount of tissue samples limits the accuracy of pathological examination to a certain extent. Sometimes only routine microscopic examination can be performed but immunohistochemistry and gene mutation detection cannot be performed, which limits the accuracy of diagnosis. sex, unable to guide subsequent treatment

[0005] 2) Insufficiency of conventional decalcification methods: decalcification is an important part of making bone tissue slices, especially bone tissue that needs to be immunohistochemically stained

At present, there is an improved EDTA-microwave radiation combined decalcification method, which can shorten the decalcification time, but it is difficult to control the heating time when using this method, and the procedure is cumbersome, and it is easy to cause damage to the specimen due to excessive decalcification

[0006] 3) There are no bone-specific immunohistochemical indicators: There are many primary tumors derived from bone metastases, and ordinary pathological results can only confirm the diagnosis of metastatic cancer in most cases, and rarely identify the primary cancer site. Immunohistochemical examination Information on some primary tumors is available, but currently there is no standard immunohistochemical panel for prognostic prediction

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