Diagnostic system for bone metastasis cancer and operation method thereof

A technology for bone metastases and a diagnosis system, applied in the field of bioimmunology, can solve the problems of complicated procedures, destruction of specimens, rarely being able to identify the primary cancer site, etc., and achieve the effects of reducing side effects and improving the positive rate.

Inactive Publication Date: 2017-11-10
赵晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) Insufficiency of bone puncture: bone puncture is the first step in obtaining tissue samples, but as an invasive operation, it is not widely carried out in hospitals at all levels
The amount of tissue samples obtained by bone biopsy is small, and the amount of tissue samples limits the accuracy of pathological examination to a certain extent. Sometimes only routine microscopic examination can be performed but immunohistochemistry and gene mutation detection cannot be performed, which limits the accuracy of diagnosis. sex, unable to guide subsequent treatment
[0005] 2) Insufficiency of conventional decalcification methods: decalcification is an important part of making bone tissue slices, especially bone tissue that needs to be immunohistochemically stained
At present, there is an improved EDTA-microwave radiation combined decalcification method, which can shorten the decalcification time, but it is difficult to control the heating time when using this method, and the procedure is cumbersome, and it is easy to cause damage to the specimen due to excessive decalcification
[0006] 3) There are no bone-specific immunohistochemical indicators: There are many primary tumors derived from bone metastases, and ordinary pathological results can only confirm the diagnosis of metastatic cancer in most cases, and rarely identify the primary cancer site. Immunohistochemical examination Information on some primary tumors is available, but currently there is no standard immunohistochemical panel for prognostic prediction

Method used

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  • Diagnostic system for bone metastasis cancer and operation method thereof
  • Diagnostic system for bone metastasis cancer and operation method thereof
  • Diagnostic system for bone metastasis cancer and operation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] This embodiment is the bone puncture needle in the bone puncture system of the present invention.

[0079] Such as figure 2 and image 3 As shown, the above-mentioned bone puncture needle includes a needle tube 2 and a bone puncture needle handle 1 affixed thereto. The needle tube 2 is sequentially sleeved with an extractor 6 and a needle core 4, and a cavity is provided in the center of the upper part of the bone puncture needle handle 1. For placing the stacked extractor handle 5 and needle core handle 3, the extractor handle 5 is fixedly connected to one end of the extractor 6, and the needle core handle 3 is fixedly connected to one end of the needle core 4, The core handle 3 is movably connected to the bone needle handle 1 (such as buckle connection / sleeve connection / snap connection), wherein the extractor 6 has convergent C-shaped claws. The above-mentioned connection between the bone needle handle 1 and the needle core handle 3 can fix the extractor handle 5 b...

Embodiment 2

[0082] This embodiment is a diagnosis system for bone metastases according to the present invention.

[0083] Such as figure 1 As shown, the above-mentioned diagnosis system for bone metastases includes a bone puncture system, a bone tissue section preparation system, an immunohistochemical kit, and a protein detection system. In the bone puncture system, a CT scanner is used to guide a bone puncture needle to perform bone puncture. It includes a guiding system (such as a puncture navigation device and a positioning device, etc.), and the bone puncture needle used includes an extractor with a convergent C-shaped claw to facilitate the extraction of tissue samples; All reagents and tools for tissue section preparation, the reagents include neutral formalin solution and EDTA decalcification solution, etc., wherein EDTA decalcification solution is 10% EDTA solution, and its components and contents are as follows: EDTA 400g, NaOH 170g- 174g, 4L of distilled water, the tools inclu...

Embodiment 3

[0085] This embodiment is an operation method for non-diagnostic and non-therapeutic purposes using the diagnosis system for bone metastases described in Embodiment 2.

[0086] The first step: bone puncture sample extraction step;

[0087] Before biopsy, X-ray or CT or MRI or PET / CT imaging examination is performed to select the lesion to be punctured and simulate the puncture path. The puncture is performed under the guidance of a CT scanner, and local anesthesia is given before the operation.

[0088] (1) Sampling of solid tissue in bone: if image 3 As shown, after the percutaneous needle is inserted, rotate the needle, pull out the needle core after penetrating the cortical bone, and continue to rotate the needle until the lesion is wedged into the needle tube in a columnar shape, first horizontally displace the extractor handle, and then vertically displace the extractor handle , press hard to the end, at this time the lesion columnar tissue is restrained by the conve...

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Abstract

The invention discloses a diagnostic system for bone metastasis cancer. The diagnostic system includes a bone puncture system, a bone tissue section preparation system, an immunohistochemistry kit and a protein detection system. The invention further relates to an operation method of the diagnostic system. The method includes the steps of under the guidance of a CT scanner, using a medullo-puncture needle to conduct bone puncture to obtain specimens; conducting decalcification treatment on the obtained specimens by using an EDTA decalcifying solution, and making bone tissue sections; conducting immunohistochemistry preparation by using the immunohistochemistry kit, and conducting standardized detection on different primary tumor sources; conducting prognosis correlation detection by using the protein detection system. The diagnostic system for bone metastasis cancer is optimized overall in process, reagent and device, plenty of specimens are extracted, and the preparation process of the bone tissue sections is shortened; therefore, it is ensured that immunohistochemistry and gene detection can be well conducted on the specimens, and the standardized detection of the different primary tumor sources and prognosis correlation protein detection can be achieved, so that the accuracy of the diagnose is improved.

Description

technical field [0001] The invention belongs to the technical field of biological immunology, and in particular relates to a diagnosis system for bone metastases and an operation method thereof. Background technique [0002] The diagnosis of bone metastases is divided into clinical diagnosis and pathological diagnosis. Pathological diagnosis is the gold standard. Bone tissue needs to be obtained. After making pathological sections, pathological morphology, immunohistochemistry, and gene mutation detection are performed to finally identify bone metastases and guide subsequent treatment. [0003] The deficiencies of commonly used techniques for clinical pathological diagnosis include: [0004] 1) Insufficiency of bone puncture: Bone puncture is the first step in obtaining tissue samples, but as an invasive operation, it is not widely carried out in hospitals at all levels. The amount of tissue samples obtained by bone biopsy is small, and the amount of tissue samples limits ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N1/30G01N1/44G01N33/68
CPCG01N33/574G01N1/30G01N1/44G01N33/68
Inventor 赵晖张惠箴顾一峰王智煜刘亮
Owner 赵晖
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