Molecular detection method for prunusmume flower bud dormancy state

A dormant state, flower bud technology, applied in the field of molecular biology, can solve problems such as ambiguity
CN107345247AActive Publication Date: 2017-11-14BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2017-11-14

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Abstract

The invention relates to the field of molecular biology and in particular discloses application of Pm011905 genes (SEQ ID NO.1) in an aspect of monitoring a prunusmume flower dormancy state and a molecular detection method for a prunusmume flower bud dormancy state. The method comprises the following steps: (1) collecting flower buds of to-be-detected prunusmume flower twice, respectively extracting RNA of the flower bud and performing inverse transcription to obtain cDNA, and by taking the cDNA as a template, carrying out a fluorescent quantitative PCR (Polymerase Chain Reaction) on Pm011905 genes by utilizing primers shown as SEQ ID NO.2-3; and (2) comparing the expression quantity of the Pm011905 genes of the flower buds collected twice, and judging the dormancy stage of the prunusmume flower buds. The method disclosed by the invention first discovers that the dormancy state of the flower buds can be monitored or judged by virtue of the change of the expression quantity of the Pm011905 genes of the flower buds. The invention provides a simple, convenient, rapid and accurate method capable of detecting the prunusmume flower bud dormancy state on a molecular level.
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Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a method for detecting the dormant state of plum flower buds by molecular markers. Background technique

[0002] Dormancy is a beneficial biological characteristic, and it is a biological adaptation to environmental conditions and seasonal changes obtained by higher plants through long-term evolution. The induction, maintenance, and release of flower bud dormancy are a complex and continuous biological process, which is the result of the interaction between plant internal regulation and external environmental factors. According to the inducement of dormancy, Lang (1987) called the temporary cessation of visible growth of plant structures containing meristems as dormancy, and divided them into para-dormancy, endo-dormancy and ecological dormancy ( eco-dormancy) and divided the whole process of dormancy into five stages: dormancy-like, dormancy-like-endormancy, endormancy, endorma...

Claims

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