Unlock instant, AI-driven research and patent intelligence for your innovation.

Analysis method, analysis reagent, analysis kit, and test strip for analysis of glycated protein

A technology for analyzing reagents and kits, which is applied in the field of analysis of glycosylated proteins, can solve the problems of complicated operations, turbidity of reaction solution, troublesome cost, etc., and achieve the effect of simple and accurate analysis

Active Publication Date: 2022-02-11
ARKRAY INC
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the first method, two reaction systems (two reaction systems) using different reagents are required for the sample, which is troublesome and costly.
In addition, in the case of the second method, the use of uricase causes the reaction solution to become cloudy, which affects the measurement of absorbance. In addition, in the use of the above-mentioned glycosaminoglycan as an insoluble carrier, it needs to be immobilized. , the operation becomes complicated due to cleaning, reaction stop, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Analysis method, analysis reagent, analysis kit, and test strip for analysis of glycated protein
  • Analysis method, analysis reagent, analysis kit, and test strip for analysis of glycated protein
  • Analysis method, analysis reagent, analysis kit, and test strip for analysis of glycated protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] With regard to the analysis method of glycated protein of the present invention, it was confirmed that the concentration of glycated protein can be measured without being affected by uric acid, which is a reducing substance in blood, by measuring fructosamine.

[0121] (1) Sample

[0122] Serum 1 (uric acid not added-) with a fructosamine concentration of 198.5 μmol / L and serum 2 (uric acid not added-) with a fructosamine concentration of 503 μmol / L were prepared, and the serum 1 and serum 2 were prepared at 20 mg / 100 mL Serum 1 (uric acid added +) and serum 2 (uric acid added +) added with uric acid were used as serum samples.

[0123] (2) Production of analytical reagent sheets

[0124] Using the first liquid reagent and the second liquid reagent of the following composition, the above-mentioned figure 1 The test piece for analysis of the example shown.

[0125]

[0126] Sodium carbonate buffer (pH11.0) 500mmol / L

[0127] (manufactured by Wako Pure Chemical Indu...

Embodiment 2

[0163] In the method for analyzing glycated protein of the present invention, it was confirmed by measuring fructosamine that the concentration of glycated protein can be measured without being affected by uric acid, which is a reducing substance in blood, at various concentrations of WST-9.

[0164] As Example 2, the concentration of the chromogenic agent WST-9 in the above-mentioned second liquid reagent is respectively set to 1mmol / L, 2.5mmol / L, 5mmol / L, 7.5mmol / L, 10mmol / L and 20mmol / L , except that, in the same manner as in Example 1 (2) above, six types of analytical test pieces 1 having different concentrations of WST-9 were produced. In addition, as serum samples, serum 4 (uric acid not added-) having a fructosamine concentration of 220.3 μmol / L and serum 5 (uric acid not added-) having a fructosamine concentration of 486.5 μmol / L were prepared, and the serum 4 and the above serum Among 5, serum 4 (uric acid supplemented +) and serum 5 (uric acid supplemented +) supple...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
lengthaaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method for analyzing glycated protein, an analysis reagent, an analysis kit and a test piece for analysis. The present invention provides a method capable of more conveniently and accurately analyzing glycated proteins in a biological sample. The method for analyzing glycated protein in a biological sample of the present invention is characterized by comprising a reaction step of reacting a chromogenic agent represented by the following formula (1) with a biological sample, and measuring the chromogenic reaction of the chromogenic agent. measurement process. In the formula (1), X is an alkali metal ion.

Description

technical field [0001] The present invention relates to a method for analyzing glycated protein, an analysis reagent, an analysis kit and a test piece for analysis. Background technique [0002] Glycated protein in blood is used as an index of short-term blood sugar control. Glucose aldehyde groups are bonded to the N-terminal or amino groups of the side chains of proteins in the blood, and amadori rearrangement produces glycated proteins that are Amadori compounds. The resulting glycated protein is also known as fructosamine due to the fructose structure of the side chain. Fructosamine is a general term for glycated proteins present in the blood. [0003] Glycated protein is a reducing substance, so a substrate that develops color by reacting with a reducing substance (such as 2-(4-indolephenyl)-3-(4-nitrophenyl)-5-phenyl-2H -Tetrazolium chloride (INT) etc.) to measure glycated protein by the degree of color development of the matrix. However, the above-mentioned matrix...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N21/78G01N33/523G01N33/66G01N33/525G01N33/68G01N33/52
Inventor 中村勤滨隆志
Owner ARKRAY INC