Measuring method for in-situ growth velocity of microalgae community based on nucleobase information
An in-situ growth rate and growth rate technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of difficult field work, inability to characterize algae growth characteristics, high temporal and spatial dynamic changes, and high analysis costs, so as to achieve convenient analysis and accuracy, low cost, and simple on-site sampling
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Embodiment 1
[0017] Growth curve and growth rate of Microcystis aeruginosa under embodiment 1, different nutrient levels
[0018] The Microcystis aeruginosa (FACHB-905) used in the experiment was purchased from the freshwater algae bank of the Chinese Academy of Sciences, and the AGP artificial medium formula (TN=4.2 mg / L, TP=0.186 mg / L) was selected. Before the experiment, the algae species were repeatedly transferred and expanded for cultivation, so that the cells could grow synchronously. In order to obtain algal cell samples with different growth rates, 6 treatment groups (NaNO 3 and K 2 HPO 4 Concentrations are different, A1 to A6 are respectively 1 / 10, 1 / 4, 1, 2, 4, and 10 times of the AGP formula concentration) for algae growth potential cultivation experiments. Three parallel experiments were set up for all treatment groups. The temperature in the light incubator was set at 28±1 °C, the light intensity was 2500 lx, the light-dark cycle ratio was 14 h:10 h, and the culture was co...
Embodiment 2
[0023] Functional relationship between [U] / [T] ratio and growth rate in Example 2, Microcystis aeruginosa cells
[0024] Through the indoor cultivation experiment of algae described in Example 1, several samples of algal cells at different growth rates in the exponential growth phase can be collected in different treatment groups. algae cell samples at 60 o Dried in an oven at constant temperature to constant weight, through the algae cell sample pretreatment experiment described in the above technical scheme, the content of nucleobase [U] and [T] was determined by high performance liquid chromatography, and the base ratio [ U] / [T]. Taking the specific growth rate μ calculated in Example 1 as the abscissa, and the nucleobase ratio [U] / [T] as the ordinate, through nonlinear fitting, the relationship between the specific growth rate and the nucleobase ratio can be obtained. regression model, such as image 3 shown. Its regression equation is: y=0.183ln(20.7ln([U] / [T])) where...
Embodiment 3、2014
[0025] Example 3, Nucleobase information and in situ growth rate of cyanobacteria in the Changjiang Estuary Reservoir in October 2014
[0026] A total of 8 surface water samples from different points in Qingcaosha Reservoir were collected in October 2014. Algae cell samples were obtained by filtering through glass fiber filter membrane (0.7 μm, Whatman, GF / F, pre-burned at 450°C for 3 h). 60 in the oven o C is dried at constant temperature to constant weight, then by implementing the algae cell pretreatment experiment described in the technical scheme, and finally using high performance liquid chromatography to measure the content of uracil and thymine, and calculate the ratio of [U] / [T], Substituting it into the calculation formula: μ=0.183+ln(20.7ln([U] / [T])), so as to obtain the in-situ growth rate of algae in Qingcaosha Reservoir at each point through inversion. Table 1 shows the measured specific growth rate of algae in Qingcaosha Reservoir, and the results are 0.394~0.5...
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