A method for measuring the in situ growth rate of microalgal communities based on nucleobase information
An in-situ growth rate and growth rate technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to characterize algae growth characteristics, high spatiotemporal dynamic changes, high analysis costs, and difficult field work, etc., to achieve analysis Convenience and accuracy, simple on-site sampling, and low cost effects
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Embodiment 1
[0017] Example 1. Growth curve and growth rate of Microcystis aeruginosa under different nutrient levels
[0018] The microcystis aeruginosa (FACHB-905) used in the experiment was purchased from the freshwater algae species bank of the graduate student of Biobiology, Chinese Academy of Sciences, and the AGP artificial medium formula was selected (TN=4.2 mg / L, TP=0.186 mg / L). Before the experiment, repeat the transfer of algae species and expand the culture to make the cells grow synchronously. In order to obtain algae cell samples with different growth rates, 6 treatment groups (NaNO 3 And K 2 HPO 4 The concentration is different, A1 to A6 are respectively 1 / 10, 1 / 4, 1, 2, 4, and 10 times the concentration of the AGP formula) for algae growth potential cultivation experiment. All treatment groups were set up for 3 parallel experiments. The temperature in the light incubator was set to 28±1 ℃, the light intensity was 2500 lx, the light-dark cycle ratio was 14 h:10 h, and the cultu...
Embodiment 2
[0023] Example 2. Functional relationship between [U] / [T] ratio and growth rate in Microcystis aeruginosa cells
[0024] Through the algae indoor culture experiment described in Example 1, several algal cell samples with different growth rates in the exponential growth stage can be collected in different treatment groups. Place the algae cell sample at 60 o C Dry in an oven at a constant temperature to a constant weight. After the algal cell sample pretreatment experiment described in the above technical scheme, the content of nucleobases [U] and [T] is determined by high performance liquid chromatography, and the base ratio is calculated [ U] / [T]. Taking the specific growth rate μ calculated in Example 1 as the abscissa and the nucleobase ratio [U] / [T] as the ordinate, through nonlinear fitting, the ratio between the specific growth rate and the nucleobase ratio can be obtained Regression model, such as image 3 Shown. The regression equation is: y=0.183ln(20.7ln([U] / [T])) whe...
Embodiment 3、2014
[0025] Example 3. Nucleobase information and in situ growth rate of cyanobacteria in the Yangtze River Estuary Reservoir in October 2014
[0026] A total of 8 surface water samples at different points in the Qingcaosha Reservoir were collected in October 2014, and filtered through glass fiber membranes (0.7μm, Whatman, GF / F, 450℃ pre-burned for 3 h) to obtain algal cell samples. 60 in the oven o C dry at a constant temperature to a constant weight, and then implement the algal cell pretreatment experiment described in the technical scheme, and finally use high performance liquid chromatography to determine the content of uracil and thymine, and calculate the ratio of [U] / [T]. Substitute into the calculation formula: μ=0.183+ln(20.7ln([U] / [T])), so as to invert the in-situ growth rate of algae in Qingcaosha reservoir at each point. Table 1 shows the measured specific growth rate of algae in Qingcaosha Reservoir, and the result is 0.394~0.563 d -1 . Compared with the samples in th...
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