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Anti-oxidative stress normal temperature preservation diluent and liquid for porcine seminal fluid and application of normal temperature preservation liquid

An anti-oxidative stress, normal temperature preservation technology, applied in the field of pig semen preservation, can solve the problems of reducing the breeding rate and breeding efficiency, damage to pig sperm cells, short effective time, etc. Oxidative stress ability, preservation effect, and effect of reducing drug residues

Inactive Publication Date: 2017-11-24
ZHANGZHOU AONONG MODERN AGRI DEV CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing diluents for storing porcine semen at room temperature have a relatively short effective time, and excess free radicals generated during storage of porcine semen at room temperature will cause oxidative stress in porcine sperm cells, resulting in damage to porcine sperm cells, decreased vitality, and even death. Reduced breeding rate and breeding benefits

Method used

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  • Anti-oxidative stress normal temperature preservation diluent and liquid for porcine seminal fluid and application of normal temperature preservation liquid
  • Anti-oxidative stress normal temperature preservation diluent and liquid for porcine seminal fluid and application of normal temperature preservation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Using hydrogen peroxide (H 2 o 2 ) to treat porcine sperm cells, establish cell models of oxidative stress damage, and then study the effect of diluents on the preservation of porcine sperm cells and the effects of porcine sperm cell damage caused by oxidative stress, and observe the various characteristics of porcine semen quality under a high-power microscope The content of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in porcine cell culture medium was detected by colorimetry. The test process is as follows:

[0040] Using single factor design, 150 breeding boars with similar health status and body weight were selected and randomly divided into 6 groups, which were respectively used as the control group and experimental group 1-5. The pre-feeding period was 7 days and the test period was 30 days. After the test, the sperm motility rate, acrosome integrity rate, plasma membrane integrity rate, sperm deformity rate, sperm motility, malondialdehyde (MDA...

Embodiment 2

[0050] A pig semen anti-oxidative stress storage solution at room temperature, the storage solution is composed of a diluent and a diluent, and each 1L of the storage solution includes the following components and contents of the diluent: bovine serum albumin 1g, trishydroxymethylaminomethane 6g , cysteine ​​0.1g, sodium citrate 10g, chelating agent 2.4g, egg yolk 0.22L, trehalose 0.08L, sodium hyaluronate 2g, potassium chloride 0.5g, antioxidant 1mmol, fungicide 0.1g, left-handed meat Alkali 1g and tea polyphenols 0.5g.

[0051] Wherein, the chelating agent is ethylenediaminetetraacetic acid; the antioxidant is melatonin; the fungicide is enrofloxacin; the diluent is phosphate buffered saline.

[0052] When in use, add 3mL of preservation solution to every 1mL of porcine semen for normal temperature preservation of porcine semen.

Embodiment 3

[0054] A pig semen anti-oxidative stress storage solution at room temperature, the storage solution is composed of a diluent and a diluent, and each 1L of the storage solution includes the following components and contents of the diluent: bovine serum albumin 6g, trishydroxymethylaminomethane 5g , cysteine ​​0.15g, sodium citrate 6g, chelating agent 3g, egg yolk 0.17L, trehalose 0.12L, sodium hyaluronate 1g, potassium chloride 1.1g, antioxidant 0.5mmol, fungicide 0.2g, left-handed meat Alkali 0.2g and tea polyphenols 2g.

[0055] Wherein, the chelating agent is ethylenediaminetetraacetic acid; the antioxidant is melatonin; the fungicide is enrofloxacin; and the diluent is BTS diluent.

[0056] When in use, add 5mL of preservation solution to every 1mL of porcine semen for normal temperature preservation of porcine semen.

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Abstract

The invention relates to anti-oxidative stress normal temperature preservation diluent and liquid for porcine seminal fluid and application of the normal temperature preservation liquid. The preservation liquid contains a diluent and dilution liquid. Each 1L of the preservation liquid contains the following diluent components by weight: 1g-6g of bovine serum albumin, 5g-6g of trihydroxymethylaminomethane, 0.1g-0.15g of cysteine, 6g-10g of sodium citrate, 2.4g-3g of a chelating agent, 0.17L-0.22L of yolk, 0.08L-0.12L of mycose, 1g-2g of sodium hyaluronate, 0.5g-1.1g of potassium chloride, 0.5mmol-1mmol of an antioxidant, 0.1g-0.2g of a bactericide, 0.2g-1g of L-carnitine and 0.5g-2g of tea polyphenol. In an application process, 3mL-5mL of the preservation liquid is added into each 1mL of the porcine seminal fluid so as to realize the normal temperature preservation of the porcine seminal fluid. Compared with the prior art, the preservation diluent and the preservation liquid have the advantages that the components are mutually matched, so that porcine semen cells are protected, an appropriate living environment is provided for porcine semen, the normal physiological activity of the porcine semen cells is guaranteed, excess free radicals are effectively removed, the oxidative stress resistance and preservation effect of the porcine seminal fluid are improved, the survival rate of the porcine semen is guaranteed, and furthermore, the breeding rate and culture benefits of sows are increased.

Description

technical field [0001] The invention belongs to the technical field of porcine semen preservation, and relates to a diluent for anti-oxidative stress porcine semen preservation at room temperature, a preservation solution and applications thereof. Background technique [0002] Pork accounts for more than 60% of my country's total meat consumption, and the production status of pork directly affects people's living standards. At present, more and more meat pigs are bred through artificial insemination technology. The application of pig artificial insemination technology can reduce the amount of breeding boars, reduce breeding costs, and increase the conception rate and litter size of sows, thereby improving the economic benefits of pig farming. With the widespread application of artificial insemination technology, the preservation technology of pig semen has also been continuously developed. Preservation of pig semen refers to prolonging the survival time of pig sperm in vit...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 万露陈凌锋施建成龙毅万文峰周盛昌王丽娜
Owner ZHANGZHOU AONONG MODERN AGRI DEV CO LTD
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