Goat pox virus of tk gene deletion type and its preparation method and application

Abstract

The invention provides a TK gene deletion type goat pox virus and a preparation method thereof. The preparation method comprises the following steps: firstly, constructing two kinds of goat pox virus shuttle vectors pGVTK and pGVTKalpha, wherein core elements contained in a plasmid pGVTK are a TK gene homologous left arm, a pox virus general early and late promoter PE / L, a fluorescent marker gene, a transcription terminator and a TK gene homologous right arm sequentially, and core elements contained in a plasmid pGVTKalpha are a TK gene homologous left arm, an insignificant sequence and a TK gene homologous right arm sequentially; and then specifically knocking down a TK gene by utilizing a secondary homologous recombination technology, wherein a selection marker can be knocked down together. The toxicity of the constructed TK gene deletion type goat pox virus is reduced to some extent, but virus proliferation is not influenced, the growth characteristics of a parent strain are still kept, and the TK gene deletion type goat pox virus also has good genetic stability, is applicable to preparation of a goat pox vaccine and has good safety.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a TK gene-deleted goat pox virus and its preparation method and application. Background technique [0002] Goat pox (goat pox) is a highly contagious disease of goats, characterized by fever, generalized pox, nodules and death. Because the disease can cause serious economic losses and hinder international trade, it is listed as a reportable infectious disease by the World Organization for Animal Health (OIE), while my country's Ministry of Agriculture lists it as a first-class animal disease. [0003] Although the existing attenuated vaccine can effectively prevent the occurrence of the disease, there are still relatively large safety hazards in use, and it is difficult to distinguish vaccination from virulent infection. my country is a country where goat pox frequently occurs, and the only way to control the disease fundamentally is to start the eradication plan, so it ...

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