Diluent for long-term storage of Tibetan sheep sperm and preparation method of diluent
A long-term preservation and dilution technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, to achieve the effects of improving reproductive performance and economic benefits, improving sperm motility rate, and improving estrus conception rate
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[0021] Example 1
[0022] A diluent for long-term preservation of Tibetan sheep semen, composed of the following components: double distilled water:
[0023] 90 ml, sucrose 3 g, fructose: 0.4 g, sodium citrate: 3 g, tris: 0.1 g, vitamin B12: 3 ml, ethylene glycol: 5 ml, egg yolk: 10 ml, penicillin: 8 Ten thousand units and streptomycin: 80,000 units.
[0024] The preparation method includes the following steps:
[0025] S1: Liquid A configuration: Take a 100 ml measuring cylinder, put sucrose, sodium citrate, fructose and tris, and add 50 ml of double distilled water to it, stir with a sterilized glass rod, and wait. After dissolving completely, add double distilled water to make the volume to 100ml. After mixing, filter it in a triangular beaker with filter paper, tie the mouth of the bottle, put it in a water bath at 75℃, boil it for 30 minutes, cool it afterwards, take 85ml in In a conical flask, add egg yolk, penicillin and streptomycin, put it in a magnetic stirrer and stir tho...
Example Embodiment
[0031] Example 2
[0032] A diluent for long-term preservation of Tibetan sheep semen, composed of the following components: double distilled water:
[0033] 110 ml, sucrose 4 g, fructose: 0.6 g, sodium citrate: 4 g, tris: 0.3 g, vitamin B12: 7 ml, ethylene glycol: 15 ml, egg yolk: 20 ml, penicillin: 12 Ten thousand units and streptomycin: 120,000 units.
[0034] The preparation method includes the following steps:
[0035] S1: Liquid A configuration: Take a 100 ml measuring cylinder, put sucrose, sodium citrate, fructose and tris, and add 50 ml of double distilled water to it, stir with a sterilized glass rod, and wait. After dissolving completely, add double distilled water to make the volume to 100ml. After mixing, filter it in a triangular beaker with filter paper, tie the mouth of the bottle, put it in a water bath at 75℃, boil it for 30 minutes, cool it afterwards, take 85ml in In a conical flask, add egg yolk, penicillin and streptomycin, put it in a magnetic stirrer and stir...
Example Embodiment
[0041] Example 3
[0042] A diluent for long-term preservation of Tibetan sheep semen, composed of the following components: double distilled water:
[0043] Double distilled water: 100ml, sucrose 3.7g, fructose: 0.5g, sodium citrate: 3.7g, tris: 0.2g, vitamin B12: 5ml, ethylene glycol: 10ml, egg yolk: 15ml, Penicillin: 100,000 units and streptomycin: 100,000 units.
[0044] The preparation method includes the following steps:
[0045] S1: Liquid A configuration: Take a 100 ml measuring cylinder, put sucrose, sodium citrate, fructose and tris, and add 50 ml of double distilled water to it, stir with a sterilized glass rod, and wait. After dissolving completely, add double distilled water to make the volume to 100ml. After mixing, filter it in a triangular beaker with filter paper, tie the mouth of the bottle, put it in a water bath at 75℃, boil it for 30 minutes, cool it afterwards, take 85ml in In a conical flask, add egg yolk, penicillin and streptomycin, put it in a magnetic stir...
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