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Diluent for long-term storage of Tibetan sheep sperm and preparation method of diluent

A long-term preservation and dilution technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, to achieve the effects of improving reproductive performance and economic benefits, improving sperm motility rate, and improving estrus conception rate

Pending Publication Date: 2017-11-28
青海省家畜改良中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because cryogenic freezing damages sheep sperm more severely than bovine and goat sperm, about 40-50% of sheep sperm are viable after freezing-thawing, but only 20-30% remain biologically intact Sex, that is, less than 30% of the sperm acrosome is intact

Method used

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  • Diluent for long-term storage of Tibetan sheep sperm and preparation method of diluent
  • Diluent for long-term storage of Tibetan sheep sperm and preparation method of diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A diluent for long-term preservation of Tibetan sheep semen, consisting of the following components: double distilled water:

[0023] 90 ml, sucrose 3 g, fructose: 0.4 g, sodium citrate: 3 g, tris: 0.1 g, vitamin B12: 3 ml, ethylene glycol: 5 ml, egg yolk: 10 ml, penicillin: 8 10,000 units and streptomycin: 80,000 units.

[0024] Its preparation method comprises the following steps:

[0025] S1: Liquid A configuration: Take a 100ml graduated cylinder, put sucrose, sodium citrate, fructose and trishydroxymethylaminomethane into it, and add 50ml of double distilled water to it, stir with a sterilized glass rod, wait until After completely dissolving, add double distilled water to make the volume to 100ml, mix well, filter it with filter paper, put it in a triangular beaker, tie the mouth of the bottle, put it in a water bath at 75°C and boil it for 30 minutes, cool it after the end, take 85ml in In the Erlenmeyer flask, add egg yolk, penicillin and streptomycin, fully s...

Embodiment 2

[0032] A diluent for long-term preservation of Tibetan sheep semen, consisting of the following components: double distilled water:

[0033] 110ml, sucrose 4g, fructose: 0.6g, sodium citrate: 4g, tris: 0.3g, vitamin B12: 7ml, ethylene glycol: 15ml, egg yolk: 20ml, penicillin: 12 10,000 units and streptomycin: 120,000 units.

[0034] Its preparation method comprises the following steps:

[0035]S1: Liquid A configuration: Take a 100ml graduated cylinder, put sucrose, sodium citrate, fructose and trishydroxymethylaminomethane into it, and add 50ml of double distilled water to it, stir with a sterilized glass rod, wait until After completely dissolving, add double distilled water to make the volume to 100ml, mix well, filter it with filter paper, put it in a triangular beaker, tie the mouth of the bottle, put it in a water bath at 75°C and boil it for 30 minutes, cool it after the end, take 85ml in In the Erlenmeyer flask, add egg yolk, penicillin and streptomycin, fully stir i...

Embodiment 3

[0042] A diluent for long-term preservation of Tibetan sheep semen, consisting of the following components: double distilled water:

[0043] Double distilled water: 100ml, sucrose 3.7g, fructose: 0.5g, sodium citrate: 3.7g, tris: 0.2g, vitamin B12: 5ml, ethylene glycol: 10ml, egg yolk: 15ml, Penicillin: 100,000 units and streptomycin: 100,000 units.

[0044] Its preparation method comprises the following steps:

[0045] S1: Liquid A configuration: Take a 100ml graduated cylinder, put sucrose, sodium citrate, fructose and trishydroxymethylaminomethane into it, and add 50ml of double distilled water to it, stir with a sterilized glass rod, wait until After completely dissolving, add double distilled water to make the volume to 100ml, mix well, filter it with filter paper, put it in a triangular beaker, tie the mouth of the bottle, put it in a water bath at 75°C and boil it for 30 minutes, cool it after the end, take 85ml in In the Erlenmeyer flask, add egg yolk, penicillin and...

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Abstract

The invention discloses a diluent for long-term storage of Tibetan sheep sperm. The diluent is prepared from the following components: 90-110 ml of double-distilled water, 3-5 g of saccharides, 3-4 g of a buffering agent, 0.1-0.3 g of tris(hydroxymethyl)aminomethane, 3-7 ml of vitamin B12, 5-10 ml of an osmotic protective agent, 10-20 ml of egg yolk, 80,000-120,000 units of penicillin and 80,000-120,000 units of streptomycin. A preparation process of the diluent comprises the following steps: S1, preparation of a solution A; S2, preparation of a solution B; S3, preparation of ethylene glycol, S4, dilution of the sperm, S5, balancing; S6, freezing. The diluent in the formula has the effects of improving sperm motility, acrosome intact rate and cycle conception rate after cryopreservation, the cycle conception rate of efficient cultivation and artificial insemination of Tibetan sheep is increased, reproductive performance and economic benefit of the Tibetan sheep are remarkably improved, and the breed conservation effect of Tibetan sheep breed resources is realized.

Description

technical field [0001] The invention relates to the technical field of Tibetan sheep semen diluent, in particular to a diluent for long-term storage of Tibetan sheep semen and a preparation method thereof. Background technique [0002] Semen cryopreservation is a key technical link in the artificial insemination technology of Tibetan sheep. It solves the problem of long-term storage of semen, makes semen not limited by time, region, and the lifespan of breeding stock, facilitates provincial and international exchanges, maximizes the use of high-quality rams, and accelerates Breeding and improvement of breeds. At the same time, it is of great significance to carry out short-term descendant determination of excellent breed rams, retain and restore the characteristics of excellent genes of Tibetan sheep breeds or individual rams, and have great significance in the aspects of bloodline renewal, introduction, conservation, and reduction of production costs. The research on shee...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 张晋青马元梅莫延新胡宁玺常永梅赵静袁海洲谢秀梅张子安谭建宁杨玉芹李景池胜刚
Owner 青海省家畜改良中心
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