Dairy cow nipple medicated bath agent as well as preparation method and application thereof
A technology for dairy cow teats and medicated bath agent, which is applied in the field of dairy cattle breeding, can solve the problems of affecting milk quality and food safety, short duration of action and high cost, and can prevent dairy cow mastitis, chapped and frostbite, and solve high-cost problems. Effect
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Embodiment 1
[0022] Example 1: Preparation of acidic electrolyzed water
[0023] According to the mass ratio of tap water and sodium chloride 1:0.5%, mix well, stir well, and then place it in the electrolytic cell of the acidic electrolyzed water generator. The area is 108cm 2 Carry out the electrolysis reaction for 20 minutes, use HCl and NaOH to adjust the pH value to 2.5-6.5, obtain the required effective chlorine concentration of 30-100 mg / L for the experiment by distilling water dilution method, and obtain the acidic electrolyzed water in the medicated bath.
[0024] The available chlorine (ACC) in acidic electrolyzed water was determined by iodometric method (American Public Health Association, 1975).
Embodiment 2
[0025] Example 2: Effect of glycerin on main physical and chemical indicators of acidic electrolyzed water
[0026] Adopt the method of Example 1 to prepare acidic electrolyzed water with available chlorine of 60 mg / L, and add 3% and 10% glycerin by volume to the above-mentioned acidic electrolyzed water, store in a 1.5L plastic bottle, and place the plastic bottle indoors. The available chlorine and pH were measured at 0h, 0.05h (3min), 0.5h, 1h, 2h, 6h, 12h, and 24h respectively.
[0027] Such as figure 1 As shown, the available chlorine content of acidic electrolyzed water added with glycerol gradually decreased with the prolongation of storage time. After standing for 2 hours, the available chlorine of acidic electrolyzed water with 3% glycerin dropped from 59.80 mg / L to 34.27 mg / L, a loss of 43%; the available chlorine of acidic electrolyzed water with 10% glycerin dropped from 59.80 mg / L to 25.64 mg / L, a loss of 57%; the loss of acidic electrolyzed water added with dif...
Embodiment 3
[0030] Example 3: In vitro simulated sterilization experiment
[0031] 1. Test strain
[0032] Escherichia coli (Escherichiacoli), Staphylococcus aureus (Staphylococcus aureus), Streptococcus agalactiae (Streptococcus agalactiae), Streptococcus dysgalactiae (Streptococcusdysgalactiae) are the main pathogenic bacteria that cause mastitis in cows. Escherichia coli and Staphylococcus aureus were provided by the Veterinary College of Inner Mongolia Agricultural University; Streptococcus agalactiae (CVCC3940) and Streptococcus dysgalactiae (CVCC3701) strains were purchased from China Veterinary Microbiology Collection Management Center.
[0033] 2. Configuration of medicated bath agent
[0034] Acidic electrolyzed water was prepared by the method in Example 1.
[0035] Acidic electrolyzed water-1: ACC: 30mg / L, pH: 5.5-6.5, hereinafter referred to as: AEW (30).
[0036] Acidic electrolyzed water-2: ACC: 60mg / L, pH: 5.5-6.5, hereinafter referred to as: AEW (60).
[0037] AEW (60)...
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