Suspension cell culture bottle

Abstract

The invention discloses a suspension cell culture bottle which comprises a bottle body, a bottle opening, a bottle cover, a plurality of vent holes, a fixing plate, a fixing plate driving mechanism, moveable plates and moveable plate driving mechanisms, wherein the plurality of the vent holes are formed in the bottle cover; when the bottle cover is screwed tightly, two moveable plates are withdrawn inwards, so that cells are moved upwards from two sides of the bottle body when being suspended gradually, and thus suspension culture is achieved; when the bottle cover is opened, the two moveable plates are driven by the moveable plate driving mechanisms to expand to the left side and the right side relative to the fixing plate; two rubber strips III are in sealing contact with the left side surface and the right side surface of the bottle body respectively. Cells suspended at the upper end in the bottle body are sealed and partitioned by using the fixing plate and the moveable plates through a rubber strip I, a rubber strip II and a rubber strip III, a culture solution at the lower half part of the bottle body is poured out from the bottle opening after passing through a bracket, and a new culture solution is added from the bottle opening. The phenomenon of cell contamination is avoided, the culture solution is saved, and the efficiency of replacing the culture solution is improved.

Description

technical field [0001] The invention relates to the field of cell culture experiment equipment, in particular to a suspension cell culture bottle. Background technique [0002] Cell culture usually changes the medium once every 2-3 days, and the process of changing the medium for suspended cells is as follows: take out the cell culture bottle from the cell culture incubator, put it into a sterile operating table, suck up the cells and liquid in the bottle with a straw, transfer it to a centrifuge tube, and centrifuge , absorb and discard the supernatant, add new medium, (if the cells are subcultured, after this step - resuspend the cells, adjust the density, and then add separately...) Add the culture bottle containing new serum and medium , placed in an incubator to continue culturing. For the culture of suspended stem cell spheres, the method of changing the medium in half is used. It is necessary to erect the culture bottle and let it stand for half an hour to allow the ...

AI Technical Summary

Problems solved by technology

During this process, the processes such as pipette suction and centrifuge tube centrifugation are easy to consume cells, making their regrowth slow down; in addition, the centrifuged cells need to take out the culture bottle from the aseptic operating table, and then return to the aseptic operating table after centrifugation. The risk of contaminating the cells, once contaminated, can cause them to die; repeated cultures will increase the cost of reagent consumables, and will virtually affect the progress of the experiment and prolong the total time of the experiment

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