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Applications of Bacillus cereus CGMCC0601 fermentation broth in preparation of drugs used for treating diabetic wounds

A technology of Bacillus cereus and fermentation liquid, which is applied in the field of preparation of medicines for treating diabetic wounds, can solve the problems of limited wound repairing effect of diabetic wounds, no obvious increase in wound repair, and no effective method for treating diabetic foot, etc. To achieve the effects of accelerated healing and repair treatment, improved microcirculation, and stable quality

Inactive Publication Date: 2017-12-15
卢筱桂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the healing process of traumatic wounds in the human body, when trauma occurs in a normal healthy human body, it only needs to be applied or taken orally with corresponding wound medicines that can sterilize, prevent infection, and debride and disinfect. However, this kind of wound medicine has no obvious effect on the wound repair of cells and substances in the human body. Therefore, traditional wound medicine has very limited effect on the wound repair of diabetic wounds.
[0006] Based on the above reasons, there is currently no effective method for the treatment of diabetic wounds and complications caused by diabetes, such as ulcers, erosions, and diabetic feet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This example provides the application of a biological preparation in the preparation of a drug for treating diabetic wounds, the biological preparation contains the fermentation broth of Bacillus cereus CGMCC0601. The specific preparation steps of the biological preparation are:

[0025] 1. Preparation of CGMCC0601 strain:

[0026] S1. Preparation of mother seed culture medium: take 8 g of beef extract, 4 g of peptone, 8 g of sucrose, 15 g of agar, 2 g of sodium chloride and 1000 ml of water, adjust the pH to 7 after mixing, sterilize for 30 minutes, and prepare a mother seed culture medium plate;

[0027] S2. Inoculation: Streak the microorganism CGMCC0601 on the mother seed medium plate, cultivate it at 28° C. for 25 hours, and select a single colony with large shape and neat edges as the production strain;

[0028]S3. Prepare the required CGMCC0601 bacterial strain: Weigh 8g of beef extract, 4g of peptone, 8g of sucrose, 15g of agar, 2g of sodium chloride and 1000ml...

Embodiment 2

[0038] This example provides the application of a biological preparation in the preparation of a drug for treating diabetic wounds, the biological preparation contains the fermentation broth of Bacillus cereus CGMCC0601. The specific preparation steps of the biological preparation are:

[0039] 1. Preparation of CGMCC0601 strain:

[0040] S1. Preparation of mother seed culture medium: Weigh 10 g of beef extract, 5 g of peptone, 10 g of sucrose, 18 g of agar, 2.5 g of sodium chloride and 1000 ml of water, adjust the pH to 7.3 after mixing, sterilize for 40 minutes, and prepare a mother seed culture medium plate;

[0041] S2. Inoculation: Streak the microorganism CGMCC0601 on the parent seed medium plate, culture it at 29°C for 22 hours, and select a single colony with large shape and neat edges as the production strain;

[0042] S3. Prepare the required CGMCC0601 strain: weigh 10g of beef extract, 5g of peptone, 10g of sucrose, 18g of agar, 2.5g of sodium chloride and 1000ml o...

Embodiment 3

[0052] This example provides the application of a biological preparation in the preparation of a drug for treating diabetic wounds, the biological preparation contains the fermentation broth of Bacillus cereus CGMCC0601. The specific preparation steps of the biological preparation are:

[0053] 1. Preparation of CGMCC0601 strain:

[0054] S1. Preparation of mother seed culture medium: take 12g of beef extract, 6g of peptone, 12g of sucrose, 20g of agar, 4g of sodium chloride and 1000ml of water, adjust the pH to 7.5 after mixing, sterilize for 45min, and prepare the mother seed culture medium plate;

[0055] S2. Inoculation: Streak the microorganism CGMCC0601 on the mother seed medium plate, cultivate it at 30°C for 20 hours, and select a single colony with large shape and neat edges as the production strain;

[0056] S3. Prepare the required CGMCC0601 bacterial strain: Weigh 12g of beef extract, 6g of peptone, 12g of sucrose, 20g of agar, 4g of sodium chloride and 1000ml of ...

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Abstract

The invention belongs to the technical field of diabetic wound treatment, and more specifically provides applications of a biological preparation containing Bacillus cereus CGMCC0601 fermentation broth in preparation of drugs used for treating diabetic wounds, and a preparation method of the biological preparation. The beneficial effects are that: applications of the biological preparation containing Bacillus cereus CGMCC0601 fermentation broth in preparation of drugs used for treating diabetic wounds are disclosed for the first time, the biological preparation possesses excellent effect in treating diabetic wounds and promoting wound healing, curative effect is excellent and achieved quickly. The biological preparation with high and stable quality can be obtained via the preparation method, and treatment effect on diabetic wounds is improved further.

Description

technical field [0001] The invention belongs to the technical field of treating diabetic wounds, and in particular relates to the application of a biological preparation containing fermentation broth of Bacillus cereus CGMCC0601 in the preparation of medicines for treating diabetic wounds. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia caused by defective insulin secretion or its biological action, or both. Blood sugar is the energy supply substance in the human body. If it cannot enter the relevant tissue cells immediately and stay in the blood for a long time, it will cause serious damage to the human body. Long-term high levels of blood sugar can cause chronic damage and dysfunction to the eyes, kidneys, heart, blood vessels, and nerves. The incidence of diabetes in my country is on the rise. Up to now, the incidence of diabetes in my country has reached 11.6%. Diabetes has become the seventh leading cause ...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61P17/02A61P3/10
Inventor 卢筱桂
Owner 卢筱桂
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