A metabolic pathway for biotransformation of methanol

A biotransformation, methanol technology, applied in biochemical equipment and methods, oxidoreductase, plant genetic improvement, etc., can solve problems such as inability to transfer, unsuitable for industrial production, and complex metabolic processes

Active Publication Date: 2020-10-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In nature, methylotrophic bacteria mainly oxidize methanol through the RuMP pathway and the Serine cycle. The metabolic process of this pathway is complex and requires the participation of multiple enzymes. It has poor portability and cannot be transferred to model strains, resulting in limited application and is not suitable for industrial production.

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  • A metabolic pathway for biotransformation of methanol
  • A metabolic pathway for biotransformation of methanol
  • A metabolic pathway for biotransformation of methanol

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Experimental program
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Embodiment Construction

[0025] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0026] 1. Training method

[0027] 1.1 Medium

[0028] Seed medium (g / L): peptone 10, yeast powder 5, sodium chloride 10 (pH=7.0).

[0029] Fermentation medium (g / L): NaHPO 4 .12H2O 17.1, KH 2 PO 4 3. NaCl 0.5, NH4Cl 1

[0030] MgSO 4 0.12, CaCl 2 0.01, Methanol 3.2 (pH=7.0).

[0031] 1.2 Sterilization method

[0032] The seed medium and fermentation medium were sterilized at 121°C for 20 min, respectively, and the pH of the medium was adjusted to 7.0 with 4M NaOH before inoculation. MgSO 4 and CaCl 2 Sterilization needs to be configured separately; methanol cannot be sterilized by autoclaving, and the method of filter sterilization is used.

[0033] 1.3 Culture method

[0034] Take the glycerin tube of engi...

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Abstract

The invention provides a metabolic pathway for bio-converted methyl alcohol. The metabolic pathway for methyl alcohol is artificially constructed in escherichia coli. The metabolic pathway is simple and can enter into the central metabolic pathway of the escherichia coli within two steps. The key enzyme, namely, formaldehyde lyase (formolase) on the pathway is subjected to orthogenesis, the beneficial mutant clone is acquired in the manner of screening through whole-cell catalysis, the enzyme activity of the formaldehyde lyase is increased, the related gene on the metabolic pathway is assembled for acquiring methyl alcohol, the engineering bacteria is utilized to culture in a culture medium taking methyl alcohol as a unique carbon source and the efficient utilization of the methyl alcohol is realized in the manners of optimizing culture medium and knocking out gene.

Description

technical field [0001] The invention belongs to the technical field of biotransformation synthesis, and in particular relates to a biotransformation methanol metabolism pathway. Background technique [0002] Methanol is the simplest monohydric alcohol. It is a colorless, odorless, volatile liquid with an alcohol smell. In recent years, the production of methanol has increased year by year, and in 2014 it exceeded 65 million tons. The price of methanol has been decreasing year by year. The production of methanol has increased, and the price has become more and more convenient. However, methanol is highly toxic and easy to absorb water. It cannot be mixed with gasoline and other fuels. It cannot be transported by the original oil pipeline during transportation, and the transportation cost is very high. So convert methanol to more valuable compounds. Now chemical methods are used to synthesize methanol into other compounds, which consumes a lot of energy and pollutes the envi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/70C12N15/60C12N15/53
CPCC12N9/0006C12N9/88C12N15/70C12Y101/01244
Inventor 张燎原何远志郭泽望高慧芳杨天行李立志柳陈军
Owner FUJIAN AGRI & FORESTRY UNIV
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