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Making method of common morel liquid spawn

A technology of liquid strains and production methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of long production cycle and low output, and achieve increased income, easy dispersion, and shortened preparation time Effect

Inactive Publication Date: 2017-12-26
云南清湖山色农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, Morchella needs large-scale artificial planting and production, but the traditional production method not only has a long production cycle, but also has low yield, which can no longer meet the needs of production. In actual production, faster and more scientific breeding methods are needed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of hickory chick liquid strain, comprises the steps:

[0022] S1. Preparation of shake flask medium: 180 parts of land peeled, sliced ​​or shredded, mixed with 8 parts of soaked wheat straw, boiled until crisp but not rotten, filtered out solids with gauze to obtain juice, then added 18 parts of glucose, 1 part Potassium dihydrogen, 2 parts of magnesium sulfate, 1 part of peptone, and 1 part of vitamin B1 were stirred evenly and sterilized by heating to prepare a shake flask culture medium;

[0023] S2. Take out an appropriate amount of mycelium from the test tube, put it into a 1000 ml liquid bottle in a sterile environment, let it stand for 80 hours, and then the inserted mycelium will germinate and produce pure white mycelium. Stir on a magnetic stirrer, place the culture on a compound shaker with a frequency of 90-100 times / min and an amplitude of 6-10 cm to continue cultivating and shaking, and mature mycelia can be seen after 85 hours;

[002...

Embodiment 2

[0027] S1. Production of shake flask culture medium: 200 parts of land peeled, sliced ​​or shredded, mixed with 10 parts of soaked wheat straw, boiled until crisp but not rotten, filtered out solids with gauze to obtain juice, then added 20 parts of glucose, 2 parts Potassium dihydrogen, 3 parts of magnesium sulfate, 4 parts of peptone, and vitamin B1 were stirred evenly and sterilized by heating to prepare a shake flask culture medium;

[0028] S2. Take out an appropriate amount of mycelium from the test tube, put it into a 1000 ml liquid bottle in a sterile environment, let it stand for 96 hours, and then the inserted mycelium will germinate and produce pure white mycelium. Stir on a magnetic stirrer, place the culture on a compound shaker with a frequency of 90-110 times / min and an amplitude of 6-10 cm to continue cultivating and shaking, and mature mycelia can be seen after 96 hours;

[0029] S3. Put the liquid bacteria into 300 liters of water with a pH value between 6 an...

Embodiment 3

[0032] A preparation method of hickory chick liquid strain, comprises the steps:

[0033] S1. Preparation of shake flask culture medium: 220 parts of land peeled, sliced ​​or shredded, mixed with 12 parts of soaked wheat straw, boiled until crisp but not rotten, filtered out solids with gauze to obtain juice, then added 22 parts of glucose, 3 parts Potassium dihydrogen, 5 parts of magnesium sulfate, 6 parts of peptone, and 6 parts of vitamin B1 were stirred evenly and sterilized by heating to obtain a shake flask culture medium;

[0034] S2. Take out an appropriate amount of mycelium from the test tube, put it into a 1000 ml liquid bottle in a sterile environment, let it stand for 105 hours, and then the inserted mycelium will germinate and produce pure white mycelium. Stir on a magnetic stirrer, place the culture on a compound shaker with a frequency of 100-105 times / min and an amplitude of 6-10 cm to continue cultivating and shaking, and mature mycelia can be seen after 105 ...

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Abstract

The invention discloses a making method of a common morel liquid spawn. The method mainly comprises the following steps: making a shake flask culture medium, culturing a liquid parent culture medium, and culturing the spawn. The method solves the inconsistent fungus age problem of common morel, shortens the spawn production time, has the characteristics of good fluidity, easiness in dispersion, rapid germination and fungus age shortening, can shorten the production cycle, allows farmer households' products to go into the market about one month earlier, saves a lot of manpower and material resources, and improves enterprises' income.

Description

technical field [0001] The invention relates to the technical field of hickory chick strains, in particular to a preparation method of hickory chick liquid strains. Background technique [0002] Liquid strains use a liquid medium, and the strains grow by absorbing nutrients from the liquid medium. Compared with the traditional and complex solid seed production method, liquid strains not only meet the growth needs of the bacteria to the greatest extent through the control of nutrition, temperature, oxygen, pH and other environments, but also the metabolic waste gas produced by respiration can be timely Excluded, the metabolism is strong, the age of all mycelia is basically the same, and the mycelium vitality is stronger than that of solid strains. Liquid strain culture has the advantages of fast fluidity, good dispersibility, fast germination, fast mycelial growth, and early covering material surface. [0003] Morchella, also known as grass lily bamboo, is a precious medici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 曾春林李永华
Owner 云南清湖山色农业科技有限公司
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