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A mixing-centrifugal integrated rotor

A rotor and mixing technology, applied in the field of biomedicine, can solve problems such as time-consuming, secondary pollution, and complexity

Active Publication Date: 2022-03-15
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the traditional mixing and centrifuging operations in the above milk sample pretreatment are relatively complicated, time-consuming, and easily lead to secondary pollution, the present invention discloses a mixing-centrifugation integrated rotor, the purpose of which is to simplify the mixing and centrifuging operations. Steps to compress the sample pretreatment time, improve the pretreatment efficiency of the milk samples to be tested, and improve the sensitivity and accuracy of the existing colloidal gold immunoassay products for the determination of chemical pollutants in dairy products

Method used

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Examples

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Effect test

Embodiment 1

[0022] Example 1, the mixing-centrifugal integrated rotor of the present invention is applied to the pretreatment of milk samples with organic solvents

[0023] Operation steps: ① Turn on the power of the small centrifuge; ② Put the mixing-centrifugation integrated rotor into the rotor slot of the small centrifuge; ③ Add raw milk and pre-treatment organic reagents from the top of the integrated rotor; ④ After the rotor is covered, position Put the cap through the top cover of the centrifuge and buckle the positioning rod, start the centrifuge, and realize the mixing of the milk sample to be tested and the pretreatment reagent; ⑥Stop the centrifuge, open the top cover of the rotor, and absorb the supernatant for on-site detection, such as the detection of chemical pollutants in dairy products with a colloidal gold detection card.

[0024] The organic solvent refers to any organic solvent that is not miscible with water, does not miscible with the object to be tested, does not c...

Embodiment 2

[0025] Example 2, the mixing-centrifugal integrated rotor of the present invention is applied to the pretreatment of milk samples by strong acid and alkali

[0026] Operation steps: ① Turn on the power of the small centrifuge; ② Put the mixing-centrifuge integrated rotor into the rotor slot of the small centrifuge; ③ Add raw milk and pre-treatment strong acid or strong alkali reagent from the top of the integrated rotor; ④ Cover the rotor Finally, the positioning cap passes through the top cover of the centrifuge and buckles the positioning rod, and starts the centrifuge to realize the mixing of the milk sample to be tested and the pretreatment reagent; Solid-liquid phase separation of the internal mixed liquid; ⑥Stop the centrifuge, open the top cover of the rotor, and suck the supernatant to another rotor; ⑦Put the rotor containing the supernatant into the rotor slot of the small centrifuge; Add an alkaline or acid reagent to the top of the rotor to neutralize the supernatan...

Embodiment 3

[0028] Example 3, the mixing-centrifugal integrated rotor of the present invention is applied to the pretreatment of milk samples with heavy metal salts

[0029] Operation steps: ① Turn on the power of the small centrifuge; ② Put the mixing-centrifugation integrated rotor into the rotor slot of the small centrifuge; ③ Add raw milk and pre-treatment heavy metal salt reagent from the top of the integrated rotor; ④ After the rotor is covered, The positioning cap passes through the top cover of the centrifuge and the positioning rod is buckled, and the centrifuge is started to realize the mixing of the milk sample to be tested and the pretreatment reagent; ⑤ Keep the centrifuge running, release the buckle of the positioning cap to the positioning rod, and realize the mixing in the rotor ⑥Stop the centrifuge, open the top cover of the rotor, and add the heavy metal salt neutralization reagent; ⑦After repeating steps ④ and ⑤, stop the centrifuge, open the top cover of the rotor, and ...

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Abstract

The invention discloses a mixing-centrifugation integrated rotor. A matching mixing-centrifugation integrated rotor based on an existing small centrifuge is a rapid pre-processing device that combines mixing and centrifugation operations; the mixing-centrifugation integrated device is a Whether the positioning cap is buckled to the integrated structure of the positioning rod and the stirring paddle, the two states of relative motion and relative static of the stirring paddle and the rotor body are realized, and finally the mixing and stirring effect and the liquid-solid phase separation effect are integrated. . The invention improves the pretreatment efficiency of the milk sample to be tested, shortens the pretreatment time, reduces the risk of secondary pollution in the pretreatment, and can obviously improve the detection sensitivity. It can be used for rapid sample pretreatment in clinical diagnosis, inspection and quarantine, food safety testing, forensic identification, drug testing, drug testing and other fields.

Description

technical field [0001] The invention relates to a pretreatment device, in particular to a rotor integrated with a mixing operation and a centrifugal operation, and belongs to the technical field of biomedicine. Background technique [0002] In various inspection and testing practices, sample pretreatment steps are essential. Especially for samples with complex matrices, different pretreatment methods are set up for different detection instruments. The mixing and centrifugation steps are the most common and basic operations. Among them, mixing is mainly carried out on vortex and oscillation equipment to realize the mixing of different components or complete specific reactions; centrifugation mainly relies on different types of centrifuges and supporting rotors to realize the separation of different components. [0003] Taking the colloidal gold test card for detecting chemical pollutants in dairy products as an example, the sensitivity of the test card can be significantly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/38B04B1/00B04B7/00B01F27/80B01F101/23
Inventor 王升启郄志伟肖瑞孙楠溪王娈娈
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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