A compound probiotic agent for improving intestinal function of chicks and preparation method thereof
A technology of compound probiotics and intestinal function, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve problems such as threats to human health, low product quality, and many livestock and poultry diseases, and improve immune performance and growth performance, and the effect of improving intestinal function
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[0023] 1. Materials
[0024] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.
[0025] 2. Method
[0026] 2.1 Preparation of mixed bacterial solution
[0027] Weigh 25 grams of LB medium, dissolve it in 1000m L of tap water, mix well, divide it into three parts, sterilize with high pressure steam at 121°C for 20 minutes, and inoculate 5 mL of Bacillus velesii, Enterococcus and oligotrophic monosporum maltophilia Bacteria seed bacterial liquid, under the condition of 37 ℃, 120r / min, carry out expansion culture, adjust to the concentration of Bacillus Veles, Enterococcus, Stenotrophomonas maltophilia respectively 5.5-6.5×10 13 CFU / mL, 3.5-4.5×10 12 CFU / mL, 5.5-6.5×10 12 CFU / ml.
[0028] The formula of LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g.
[0029] The expanded cultured Bacillus...
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