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Method for accelerating germination of cremastra appendiculata seeds

A Rhododendron orchid and seed technology, applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient, incomplete embryo development, difficulty in large-scale production, etc., and achieve the effects of reducing pollution rate and increasing disinfection.

Inactive Publication Date: 2018-02-02
秦素梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although artificial pollination can make it bear fruit, it is very difficult to reproduce sexually because of obstacles such as incomplete development of the embryo and inhibition of the seed coat.
Although pseudobulbs can be used for artificial planting, the reproduction coefficient is extremely low and it is difficult to achieve large-scale production
At present, the research on Rhododendron orchid mainly focuses on chemical composition analysis, pharmacology and toxicology research, tissue culture and rapid propagation, etc. There are few reports on how to break through the problem of seed germination in sexual reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for accelerating the germination of rhododendron orchid seeds, the method comprises the following steps:

[0029] (1) Seed selection: choose rhododendron orchid capsules of different plants and cross-pollination and a growth period of 180 days;

[0030] (2) Seed surface treatment: After picking Rhododendron orchid capsules, wash them with running water to clean the surface, then place them in an ultra-clean workbench, soak them in 75% alcohol for 2 minutes, wash them with sterile water for 3 times, and then use plant disinfectant Soak for 5 minutes, finally wash 3 times with sterile water, dry the surface moisture with anhydrous filter paper; the plant disinfectant used is made by mixing the following raw materials in parts by weight: 0.10 parts of glycyrrhizic acid, 12 parts of lemon juice, 0.5 parts of honeysuckle leaf extract Parts, Tween-602 parts, water 75 parts;

[0031] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out...

Embodiment 2

[0034] A method for accelerating the germination of rhododendron orchid seeds, the method comprises the following steps:

[0035] (1) Seed selection: choose rhododendron orchid capsules of different plants and cross-pollination and a growth period of 180 days;

[0036] (2) Seed surface treatment: After picking Rhododendron orchid capsules, rinse the surface with running water, then place it in a super-clean workbench, soak it with 75% alcohol for 2.5 minutes, wash it with sterile water for 4 times, and then sterilize it with plants solution for 8 minutes, and finally washed 4 times with sterile water, and dried the surface moisture with anhydrous filter paper; the plant disinfectant used was mixed with the following raw materials in parts by weight: 0.12 parts of glycyrrhizic acid, 13 parts of lemon juice, honeysuckle leaf extract 0.6 parts, Tween-603 parts, water 85 parts;

[0037] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out the Rhodo...

Embodiment 3

[0040] A method for accelerating the germination of rhododendron orchid seeds, the method comprises the following steps:

[0041] (1) Seed selection: choose rhododendron orchid capsules of different plants and cross-pollination and a growth period of 180 days;

[0042] (2) Seed surface treatment: After picking Rhododendron orchid capsules, rinse the surface with running water, then place it in an ultra-clean workbench, soak it with 75% alcohol for 3 minutes, wash it with sterile water for 5 times, and then use plant disinfectant Soak for 10 minutes, and finally wash 5 times with sterile water, dry the surface moisture with anhydrous filter paper; the plant disinfectant used is made by mixing the following raw materials in parts by weight: 0.15 parts of glycyrrhizic acid, 15 parts of lemon juice, 0.8 parts of honeysuckle leaf extract Parts, Tween-604 parts, water 100 parts;

[0043] (3) Seed disinfection: cut open the capsule longitudinally with a scalpel, shake out the Rhodod...

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PUM

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Abstract

The invention provides a method for accelerating germination of cremastra appendiculata seeds and belongs to the technical field of plant tissue culture. The method comprises the steps of seed selection, seed surface treatment, seed sterilization and seed culture. Plant disinfection solution is mainly adopted for immersing in the step of seed surface treatment and seed sterilization, active ingredients of the plant disinfection solution are glycyrrhizic acid, lemon juice and honeysuckle leaf extract; lycium barbarum leaf juice and moringa oleifera leaf juice are added into a germination culture medium and used as nutrient components, and NAA and GGR6 are used as auxin. According to the method, cremastra appendiculata seeds can be germinated in advance, and the seed germination rate can beimproved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for accelerating the germination of rhododendron orchid seeds. 【Background technique】 [0002] Rhododendron orchid belongs to the Orchidaceae Rhododendron orchid, a perennial medicinal herb. Its dried pseudobulb is used as medicine. It can be used externally to treat snake bites and skin burns, and it can be used internally to fight liver cancer and breast cancer. Due to people's unrestrained excavation of rhododendron orchid medicinal materials, its wild resources are on the verge of exhaustion. Under natural conditions, rhododendron orchids can hardly pollinate and set fruit. Although artificial pollination can make it fruit, it is very difficult to reproduce sexually because of obstacles such as incomplete embryo development and seed coat inhibition. Although pseudobulbs can be used for artificial planting, the reproduction coefficient is extremely lo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦素梅
Owner 秦素梅
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