NK92 cell serum-free culture medium

A serum-free medium and cell technology, applied in the field of biomedicine, can solve the problems of poor biological safety and high cost

Inactive Publication Date: 2018-02-09
上海源培生物科技股份有限公司
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Problems solved by technology

[0003] ATCC recommends MEM-α medium + 12.5% ​​fetal bovine serum + 12.5% ​​horse serum for NK92 cell culture (refer to the culture conditio...

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  • NK92 cell serum-free culture medium
  • NK92 cell serum-free culture medium
  • NK92 cell serum-free culture medium

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Embodiment Construction

[0178] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention will be further elaborated below.

[0179] NK92 cell serum-free medium, in terms of final concentration, the formula includes: L-cysteine ​​hydrochloride monohydrate 1~1000mg / L, L-phenylalanine 1~1000mg / L, L-glutamine 1~1000mg / L, L-methionine 1~1000mg / L, L-lysine hydrochloride 1~1000mg / L, L-tyrosine disodium salt 1~1000mg / L, L-leucine Acid 1~1000mg / L, potassium chloride 1~1000mg / L, D-(+)-glucose 100~10000mg / L, HEPES 100~10000mg / L, sodium bicarbonate 100~10000mg / L, sodium bicarbonate 100~ 10000mg / L, sodium chloride 100~10000mg / L, recombinant human albumin 100~10000mg / L, poloxamer 188 100~10000mg / L, soybean protein 100~10000mg / L, yeast powder 100~10000mg / L, Recombinant human transferrin 0.1-100mg / L, insulin 0.1-100mg / L. HEPES is 4-hydroxyethylpiperazineethanesulfonic acid, and the present invention adds 4-hydroxyethy...

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Abstract

The invention relates to the field of biological medicine, and provides an NK92 cell serum-free culture medium. Based on final concentration, the NK92 cell serum-free culture medium is prepared from the following components: 1-1000 mg/L of L-cysteine hydrochloride monohydrate, 1-1000 mg/L of L-phenylalanine, 1-1000 mg/L of L-glutamine, 1-1000 mg/L of L-methionine, 1-1000 mg/L of L-lysine hydrochloride, 1-1000 mg/L of L-tyrosine disodium salt, 1-1000 mg/L of L-leucine, 1-1000 mg/L of potassium chloride, 100-10000 mg/L of D-(+)-glucose, and 100-10000 mg/L of HEPES. According to the NK92 cell serum-free culture medium, the formula of the traditional NK92 cell serum-free culture medium is optimized, the production cost is saved, and the cell culture efficiency is improved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a serum-free culture medium. Background technique [0002] NK92 cells are commonly referred to as natural killer cells. Unlike T cells and B cells, they need to go through the antigen recognition reaction of the immune system to determine the target "attack". When the infection first occurs, NK92 cells can actively attack and kill the infected target cells. So it is also called "natural killer". The target cells of NK92 cells mainly include certain tumor cells, virus-infected cells, parasites, etc. Therefore, NK cells are an important immune factor for the body's anti-tumor and anti-infection. [0003] ATCC recommends MEM-α medium + 12.5% ​​fetal bovine serum + 12.5% ​​horse serum for NK92 cell culture (refer to the culture conditions published on the ATCC website), this method contains high concentration of animal serum in the medium, poor biological safety, and higher cost. Cont...

Claims

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Application Information

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IPC IPC(8): C12N5/0783
CPCC12N5/0646C12N2500/12C12N2500/14C12N2500/16C12N2500/20C12N2500/22C12N2500/24C12N2500/25C12N2500/30C12N2500/32C12N2500/33C12N2500/34C12N2500/35C12N2500/36C12N2500/38C12N2500/40C12N2500/46C12N2500/60C12N2500/74C12N2500/90C12N2501/998
Inventor 孙奇威温韬肖杰
Owner 上海源培生物科技股份有限公司
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