A hydroxyethylation method for improving the immune activity of Hericium erinaceus polysaccharides
A technology of erinaceus erinaceus polysaccharide and hydroxyethylation, which is applied in the field of animal medicine, can solve the problems such as hydroxyethylated erinaceus erinaceus polysaccharides to improve immune activity technology, and achieve the goal of improving antigen presentation ability and improving immune activity Effect
Active Publication Date: 2020-04-17
FUJIAN AGRI & FORESTRY UNIV
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] There are domestic invention patent applications for hydroxyethyl pachymane sulfate and its preparation technology, but no invention patent application for hydroxyethylated Hericium erinaceus polysaccharides to improve immune activity
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0018] A hydroxyethylation method for improving the immune activity of Hericium erinaceus polysaccharides, specifically comprising the following steps:
[0019] (1) Dissolve 500 mg Hericium erinaceus polysaccharide in 25 mL of a mixed solution of sodium hydroxide and sodium chloride (the concentration of sodium hydroxide is 3wt%, the concentration of sodium chloride is 3wt%), stir at room temperature until dissolved, Obtain mixed solution A;
[0020] (2) Cool the mixed solution A obtained in step (1) to 0 °C under ice bath conditions, fill the container with nitrogen to exclude air, and obtain mixed solution B;
[0021] (3) Add 2.5 mL of ethylene oxide dropwise to the mixed solution B obtained in step (2), then stir and react in ice bath for 2 h, after the reaction is completed, let the reaction solution rise to room temperature naturally, and stir overnight;
[0022] (4) The next day, use 1 mol / L acetic acid to neutralize to neutral to obtain a mixed solution C, then add eth...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses a hydroxyethylation method capable of improving polysaccharide immunity activity of hericium erinaceus. The hydroxyethylation method capable of improving polysaccharide immunity activity of hericium erinaceus comprises the following steps: dissolving hericium erinaceus polysaccharide in a mixed solution of sodium hydroxide and sodium chloride, and stirring and dissolving; cooling the obtained solution to be at the temperature of 0 DEG C, feeding nitrogen and discharging air, then dropwise adding ethylene oxide and stirring and reacting under the condition of ice bath; after reaction is finished, heating the obtained solution to the room temperature, and stirring overnight; neutralizing the solution until the solution is neutral in the next day, adding ethanol and acetone, carrying out centrifugal separation after white precipitate appears, dissolving the precipitate with water, and then carrying out dialysis and freeze drying to obtain hydroxyethylated hericiumerinaceus polysaccharide. The hericium erinaceus polysaccharide is subjected to hydroxyethylation modification, thus, the immunocompetence of the hericium erinaceus polysaccharide is improved, by theprepared hericium erinaceus polysaccharide, the antigen presentation capability of DCs can be improved remarkably, and DCs cell secretion IL-12, IL-18 and IL-15 is promoted.
Description
technical field [0001] The invention belongs to the field of veterinary medicine, and in particular relates to a hydroxyethylation method for improving the immune activity of Hericium erinaceus polysaccharides. Background technique [0002] Polysaccharide is an important biological macromolecule, which participates in various physiological metabolisms in the body, and plays an important role in regulating immune function, cell recognition, transport of intercellular substances, diagnosis and treatment of cancer, etc. Studies have found that the various biological activities of polysaccharides are closely related to their structure and conformation. By means of molecular modification, changing the functional groups of polysaccharides can improve the activity of polysaccharides in a targeted manner. The current modification methods mainly include sulfation, acetylation, sulfonylation, carboxymethylation and phosphorylation, etc., all of which can improve the activity of polys...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
CPCC08B37/00
Inventor 秦韬黄一帆任喆李健马玉芳黄小红俞道进
Owner FUJIAN AGRI & FORESTRY UNIV