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Method for determining garlic pesticide residues

A technology for pesticide residues and determination methods, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of inapplicable properties, unstable organophosphorus pesticides, and the recovery rate cannot meet the requirements, and achieves the removal of sulfide interference, The effect of avoiding the production of allicin and promoting the use value well

Inactive Publication Date: 2018-02-23
GUANGXI SHATIAN XIANRENTAN AGRI INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are also reports of using silver nitrate to disperse on alumina and florisil to remove sulfide in vegetables, but silver nitrate has a strong adsorption to sulfur-containing pesticides, so that the recovery rate cannot meet the requirements
Potassium permanganate-sulfuric acid oxidation method can also remove sulfide in vegetables, but it is not suitable for organophosphorus pesticides with unstable properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The assay method of garlic pesticide residue, described method comprises the steps:

[0023] (1) randomly select 20 garlics from the garlic to be determined, randomly select 1 clove of garlic from each of the 20 garlics, and obtain 20 garlic cloves in total;

[0024] (2) Any one of the 20 garlic cloves is heated by 600W microwave for 120 seconds, and the sample is homogenized;

[0025] (2) Accurately weigh 10.0g±0.1g of the sample, add 60mL of acetonitrile and 20mL of water, stir at 30000 rpm for 2min; wash the knife head with 5mL of acetonitrile, filter with suction, wash the residue three times with 30mL of acetonitrile, add 5mL of water , to be purified;

[0026] (3) Pre-wash the C18 column with 20mL acetonitrile and 30mL pure water, purify the above-mentioned filtrate through a PSA column, and wash the residue with 15mL acetonitrile and 5mL water;

[0027] (4) For the eluent, add 10 mL of 3 mol / L phosphate buffer solution saturated with salt and 12 g of salt, shak...

Embodiment 2

[0032] The assay method of garlic pesticide residue, described method comprises the steps:

[0033] (1) randomly select 20 garlics from the garlic to be determined, randomly select 1 clove of garlic from each of the 20 garlics, and obtain 20 garlic cloves in total;

[0034] (2) any one of the 20 garlic cloves is heated for 90 seconds with a 600W microwave, and the sample is homogenized;

[0035] (2) Accurately weigh 10.0g±0.1g of the sample, add 40mL of acetonitrile and 10mL of water, stir at 20000 rpm for 5min; wash the knife head with 5mL of acetonitrile, filter with suction, wash the residue three times with 15mL of acetonitrile, add 1mL of water , to be purified;

[0036] (3) Pre-wash the C18 column with 10mL acetonitrile and 10mL pure water respectively, purify the above-mentioned filtrate through a PSA column, and wash the residue with 9mL acetonitrile and 3mL water;

[0037] (4) For the eluent, add 10 mL of 3 mol / L phosphate buffer solution saturated with salt and 8 g...

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Abstract

The invention discloses a method for determining garlic pesticide residues. The method comprises the following steps: firstly selecting 20 garlics from to-be-determined garlics randomly and selectingone clove of garlic from the 20 garlics randomly to obtain 20 garlic cloves; and then sequentially performing pretreatment on each of the garlic cloves, determining the content of the pesticide residues in each of the garlic cloves and stopping detection if the content of the pesticide residues in the cumulative three garlic cloves is determined to be unqualified, and therefore, the content of thepesticide residues in the batch of garlics is determined to be unqualified. The method disclosed by the invention has the benefits that the garlic cloves are treated by selecting microwaves; before the production of allicin, enzymes in garlic samples are inactivated, so that the allicin cannot be produced, and therefore, the removal of sulfide interference is achieved, and the recovery rate of to-be-determined analytes meets the requirements. The method disclosed by the invention is high in randomness and high in test credibility; and the determining method is simple, time and labor-saving and accurate in determination result, thereby having good popularization and use values.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a method for measuring pesticide residues in garlic. Background technique [0002] In the process of garlic planting, underground pests are seriously harmful, causing vegetable farmers to use a large amount of highly toxic pesticides, causing serious pollution to garlic. When people eat, pesticides may enter the human body and accumulate gradually, eventually affecting people's health. [0003] In the detection of pesticide residues in vegetables, sulfur-containing vegetables such as onions, garlic and leeks are a special kind of complex samples. When such vegetables are crushed during sample preparation, the active enzymes in them will release sulfides in the vegetables, resulting in a characteristic odor. These sulfides are not easy to remove, and will produce strong responses on gas chromatography electron capture detector (GC / ECD), gas chromatography flame photome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陆世吉王小群韦炜
Owner GUANGXI SHATIAN XIANRENTAN AGRI INVESTMENT CO LTD
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