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HPLC method for testing three active components in hemsleya amabilis

A technology of active ingredients and snow gall, which is applied in the field of HPLC for the determination of three active ingredients in snow gall, to achieve the effect of high efficiency and accuracy, good reproducibility, and quality assurance

Active Publication Date: 2018-03-16
SICHUAN AGRI UNIV
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  • Claims
  • Application Information

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Problems solved by technology

There is no report on the simultaneous determination of three active components in Snow Gallbladder by HPLC

Method used

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  • HPLC method for testing three active components in hemsleya amabilis
  • HPLC method for testing three active components in hemsleya amabilis
  • HPLC method for testing three active components in hemsleya amabilis

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Embodiment 1

[0039] Embodiment 1 The inventive method measures three kinds of active ingredient contents of snow gallbladder

[0040] 1. Preparation of the test solution

[0041] The tubers of snow gallbladder are dried at 60°C, crushed, and passed through a 60-mesh sieve. Accurately weigh 1.00g of snow gall powder, put it in a round-bottomed flask, accurately pipette 25.00mL of absolute ethanol, weigh it, and after reflux extraction for 3 hours, extract it with constant temperature and ultrasonic for 60 minutes, weigh its mass, and fill up the mass with absolute ethanol , Shake well, filter through a 0.22μm microporous membrane, and obtain it.

[0042] 2. Preparation of reference solution

[0043] Accurately weigh gentianin A, gentianin B, and oleanolic acid reference substances respectively, add absolute ethanol to dissolve and constant volume, and prepare mixed reference substance solutions with mass concentrations of 0.62, 0.75, and 0.70 mg / mL respectively. spare.

[0044] 3. Estab...

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Abstract

The invention provides a HPLC method for testing three active components in hemsleya amabilis. The active components are cucurbitacin, dihydrocucurbitacin and oleanolic acid; the method includes stepsof a, taking hemsleya amabilis powder to be detected; extracting by ethanol, filtering and preparing sample solution for test; b, taking cucurbitacin, dihydrocucurbitacin and oleanolic acid referencesubstance, mixing and adding ethanol to dissolve; preparing the reference substance solution; c, filling the reference substance and reference substance in a high performance liquid chromatograph; d,calculating to acquire content of three components in the hemsleya amabilis according to the detection result. The method can test cucurbitacin, dihydrocucurbitacin and oleanolic acid at the same time; the method is high-efficient and exact, and good in repeatability; the method provides a basis for the system evaluation and quality control of hemsleya amabilis; quality of the hemsleya amabilis drug material is more comprehensively guaranteed.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to an HPLC method for determining three active ingredients in snow gall bladder. Background technique [0002] Plants of the genus Hemsleya are perennial climbing herbaceous plants of the family Cucurbitaceae. According to Flora of my country, there are 31 species and 10 varieties of plants in this genus, which are mainly distributed in the southwest to south-central regions of China. The province holds 92% of the entire genus. The dried roots of various plants in this genus can be used for medicinal purposes. They are commonly used Chinese herbal medicines and national medicines by various ethnic groups in Southwest my country. For enteritis and various other infections, it has the advantages of less dosage, obvious effect, and no dependence. It has also been preliminarily judged as a drug that can replace antibiotics. [0003] Due to the differences in origin...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张利杨程姜媛媛吴一超胡青青朱柏雨周永红王晓丽杨瑞武丁春邦苟丽琼佘启惠吴忠旺
Owner SICHUAN AGRI UNIV
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