The invention provides an HPLC method for measuring three kinds of active ingredients in snow gallbladder, the active ingredients are snow gallbladder, snow gallbladder and oleanolic acid, comprising the following steps: a, taking the snow gallbladder to be tested Powder, extract with ethanol, filter, prepare need testing solution; b, get gentianin, gentalin, oleanolic acid reference substance, mix, add ethanol to dissolve, prepare reference substance solution; c, respectively The test solution and the reference solution are injected into a high-performance liquid chromatograph for detection; d, the contents of the three components in the snow gall are calculated according to the test results. The method of the present invention can simultaneously determine the three components of gentamidin, gentamidin and oleanolic acid, the method is efficient, accurate and has good reproducibility, and provides a basis for the systematic evaluation and quality control of gentamicin, and can more comprehensively Guarantee the quality of snow gall medicinal materials.