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Malate dehydrogenase diagnostic kit

A malate dehydrogenase and diagnostic kit technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral property measurement, etc., can solve the problem of long inspection time, troublesome operation, malate dehydrogenase Troubleshooting issues

Pending Publication Date: 2018-03-27
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In summary, the existing problems in the prior art are: the existing kits are too cumbersome for the diagnosis of malate dehydrogenase, the test time is long, and the operation is troublesome

Method used

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Embodiment Construction

[0056] In order to further understand the content, features, and effects of the present invention, the following embodiments are exemplified, and are described in detail below with accompanying drawings.

[0057] Attached below figure 1 The structure of the present invention will be described in detail.

[0058] The malate dehydrogenase diagnostic kit is provided with a first box body and a second box body adhered to each other; the middle of the first box body is riveted with a rotating shaft, the rotating shaft is sleeved with a turntable, and the outer side of the turntable is arranged There are several detection boxes; the inside of the detection box is provided with a medicine box;

[0059] A plurality of injection devices are clamped in the second box body, a detection pool is welded on the top of the first box body, and a medicine addition box is sleeved on the upper end of the detection pool.

[0060] Further, the inside of the detection box is filled with a hemostatic chlorin...

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Abstract

The invention belongs to the technical field of biological detection, and discloses a malate dehydrogenase diagnostic kit. The kit is provided with a first box and a second box which are adhered to each other; a rotating shaft is riveted to the middle of the first box, a turntable is arranged on the rotating shaft in a sleeving manner, and a plurality of detection boxes are arranged on the outer side of the turntable; a medicine box is arranged at the inner side of the detection box; a plurality of injection devices are clamped in the second box, a detection cell is welded at the top of the first box, and a medicine adding box is arranged on the upper end of the detection cell in a sleeving manner. The kit is high in sensitivity, strong in specificity and good in repeatability. Because thereagent is steady, easy for storage and simple and convenient to operate, and result judgment is relatively objective, the kit is suitable for detecting the activity of malate dehydrogenase in a biological sample, such as cell and tissue culture supernatant fluid and mitochondrion obtained by purification.

Description

Technical field [0001] The invention belongs to the technical field of biological detection, and particularly relates to a malate dehydrogenase diagnostic kit. Background technique [0002] Malate Dehydrogenase (MDH) is one of the key enzymes for the synthesis of malate. It catalyzes the mutual conversion of malate and oxaloacetate (OAA), and participates in many physiological metabolic pathways such as TCA cycle, C4 cycle fatty acid oxidation and respiration. Nitrogen Assimilation, etc., therefore, MDH plays an important role in the growth and development of plants. It is widely present in mitochondria and bacterial cell membranes. It is an enzyme in the tricarboxylic acid cycle. Due to different sources of enzymes, some of its properties are not exhaustive. the same. MDH plays an important role in many physiological activities of cells, including mitochondrial energy metabolism, malate-aspartate shuttle system, active oxygen metabolism and disease resistance. According to dif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32G01N21/31
CPCC12Q1/32G01N21/31
Inventor 李彬先孟宪玲张晓梅王丹峰吴翠翠孙亚臣石宏董理
Owner BEIHUA UNIV
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