Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing (S)-(+)-3-hydroxytetrahydrofuran by transforming engineering strains

A technology of hydroxytetrahydrofuran and ketotetrahydrofuran, which is applied in the field of transformation and preparation of engineering strains of 3-hydroxytetrahydrofuran, can solve the problems of low substrate concentration and limited selection range of strains, and achieve low cost, high tolerance, Environmentally friendly effect

Inactive Publication Date: 2018-04-13
安徽联创生物医药股份有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current microbial transformation process is immature, the selection range of strains is limited, and the substrate concentration is generally low. It is urgent to develop strains with high substrate tolerance, high expression of ketoreductase, or high-level specific expression.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for preparing (S)-(+)-3-hydroxytetrahydrofuran through transformation of engineering strains, comprising the following steps:

[0040] (1) Slant culture: inoculate Escherichia coli LC001 into the slant medium, and culture at 25°C for 20 hours to obtain the slant of the bacteria;

[0041] The preparation of the slant medium: yeast powder 5g / L, peptone 10g / L, NaCl 10 / L, LB agar 20g / L, NaOH solution to adjust pH value to 7.4, solvent is water; sterilized at 121°C for 20min, after cooling Make inclined plane; The bacterium colony characteristic of described Escherichia coli LC001 (purchased from Beijing Quanshijin Biotechnology Co., Ltd.): on the LB agar medium, present off-white, glossy, neat edges, moist, smooth surface, and uniform bacterium colony form;

[0042] (2) Seed culture: take a small amount of thalline from the thallus slope described in step (1) and transfer to the seed culture medium, at 25°C, the shaking table speed is 180r / min, and cultivate for 2...

Embodiment 2

[0050] A method for engineering strain transformation to prepare (S)-(+)-3-hydroxytetrahydrofuran, except that the initial concentration of 3-ketotetrahydrofuran in PBS buffer solution is 3mol / L in step (4), Others are with embodiment 1.

Embodiment 3

[0052] A method for transforming engineered strains to prepare (S)-(+)-3-hydroxytetrahydrofuran, except that in step (4) the initial concentration of 3-ketotetrahydrofuran in PBS buffer is 5mol / L, other same Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing (S)-(+)-3-hydroxytetrahydrofuran by transforming engineering strains. The method is characterized in that the (S)-(+)-3-hydroxytetrahydrofuran is preparedby taking 3-ketotetrahydrofuran as a substrate, immobilizing thallus cells, which are obtained by fermenting genetically engineered bacteria escherichia coli LC001 and can provide an enzyme continuously, through calcium alginate into a biological catalyst, and carrying out catalytic reaction. The method for preparing the (S)-(+)-3-hydroxytetrahydrofuran by transforming the engineering strains, provided by the invention, has the advantages of convenience for operation, high yield, moderate reaction conditions, environment friendliness, high catalysis specificity, high catalysis efficiency, high substrate and product tolerance and low cost, and has potential value in industrialized production.

Description

technical field [0001] The invention belongs to the technical field of biosynthesis, and in particular relates to a method for preparing (S)-(+)-3-hydroxytetrahydrofuran by transforming engineering strains. Background technique [0002] (S)-(+)-3-Hydroxytetrahydrofuran, English name: (S)-(+)-3-Hydroxytetrahydrofuran, molecular formula: C 4 h 8 o 2 , molecular weight: 88.10512); is an important intermediate in the synthesis of empagliflozin, afatinib tumor, amprenavir, fosamprenavir calcium. [0003] At present, many preparation methods of (S)-(+)-3-hydroxytetrahydrofuran have been reported at home and abroad, mainly chemical synthesis methods, which can be divided into three categories: chiral substrate synthesis; chiral catalyst asymmetric synthesis and elimination. Chiral resolution after synthesis of rotamers. The above-mentioned chemical synthesis method has achieved good results, but there are also some shortcomings, such as high requirements for raw materials for t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04C12N11/10C07D307/20C12R1/19
CPCC07B2200/07C07D307/20C12N11/10C12P17/04
Inventor 葛德培吴其华洪炯刘涛
Owner 安徽联创生物医药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com