A ratio-type oxygen sensing probe and its preparation method and application
An oxygen sensing probe and ratio-type technology, applied in the field of extracellular ratio-type oxygen sensing probe and its preparation, can solve the problem of fluorescence quantum efficiency as low as 0.001 to 0.0095, etc., and achieve stable structure and good oxygen sensitivity characteristics. , the effect of improving application efficiency
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Embodiment 1
[0098] In this embodiment, the polymer shown in P3 was synthesized through the following process:
[0099]
[0100] The preparation process specifically includes the following steps:
[0101] (1) P1 synthesis process:
[0102] 1.14mL (10mmol) of styrene, 2mL of anisole (Anisole), and 57.2mg (0.4mmol) of cuprous bromide were mixed and added to a 10mL Schlenk tube. The liquid nitrogen was frozen to a solid state and then vacuumed. After vacuuming, 35 μL (0.2 mmol) of initiator and 83.5 μL (0.4 mmol) of N,N,N,N,N-pentamethyldiethyleneamine (PMDETA) were added to the Schlenk tube. The liquid nitrogen is frozen and then vacuumed. After vacuuming, dissolve it with a hot solvent, and you can see a lot of bubbles coming out. Repeatedly freeze, vacuum, and dissolve. When there are no bubbles, fill with nitrogen, and ventilate for three times, then heat to 90°C for 10 hours under the protection of nitrogen. After the reaction, the solution was passed through a neutral alumina chromatograph...
Embodiment 2
[0108] In this embodiment, the copolymerized polymer CP with π-conjugated system was synthesized by the following process:
[0109] The preparation process specifically includes the following steps:
[0110] 5mL of 2.0M sodium carbonate aqueous solution and 20mL of THF are mutually dissolved and deoxygenated with argon for 30min. Then 9.87mg (0.00854mmol) of tetrakis(triphenylphosphine)palladium, 0.104g (0.34mmol) of 4,7-dibromobenzo[ c)-1,2,5-thiadiazole and 0.1266g (0.34mmol) 9,9-dihexylfluorene-2,7-diborate bis(1,3-propanediol) are mixed, deoxygenated for 30min, and then used argon Gas protection. After the two mixed liquids are mixed, deoxygenate with argon for 10 minutes, then seal, and condense and reflux at 80°C for 24 hours. After the reaction is complete, it is extracted with dichloromethane and water and dried, concentrated and dropped into ice methanol to obtain a yellow viscous substance. The yield is about 80%. M n(GPC) = 2400, M w(GPC) = 6300, M w / M n = 2.62. ...
Embodiment 3
[0112] The ability of the polymer prepared in Example 1 to form micelles in an aqueous solution was investigated. Since P3 contains the fluorophore OS1, its CMC can be determined by its fluorescence intensity during the formation of micelles in the aqueous solution. When micelles are not formed, the fluorescence emission of the fluorophore will be quenched by water to obtain weaker fluorescence. Once micelles are formed, the fluorescence intensity will be greatly enhanced. By testing the fluorescence intensity change of P3 at the concentration of 0.01 mg / mL to 100 mg / mL in aqueous solution, the CMC of P3 can be judged by the mutation point of fluorescence intensity. Take the concentration and the fluorescence intensity value at 650nm wavelength as the abscissa and ordinate to plot figure 1 ,by figure 1 The sudden change in fluorescence intensity is figure 1 The intersection of the two straight lines in the middle gives the CMC value of P3 0.35μg / mL.
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