Application of catalpol in promoting the differentiation of neural stem cells into oligodendrocytes
A technology of oligodendrocytes and neural stem cells, applied in the field of stem cell therapy, can solve the problems of unclear regulatory mechanism, lack of control of NSCs differentiation and growth, and unclear NSCs differentiation conditions, etc., to achieve drug concentration, real-time observation, The effect of saving experiment time
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[0032] Application of catalpol in promoting the differentiation of NSCs into oligodendrocytes.
[0033] 1 Design and fabrication of microfluidic chip
[0034] 1) For the study of the application of catalpol in promoting the differentiation of NSCs into oligodendrocytes, the schematic diagram of the microfluidic chip device used is as follows figure 1 shown. The whole device is composed of an upstream concentration gradient generator 1 and a downstream cell culture module 2 . Due to the ability to perform integrated operations in the chip (gradient concentration generation, stimulation and cell seeding, culturing, etc.), multiple cellular responses stimulated by different drug concentrations can be recorded simultaneously. The concentration gradient generator 1 includes a chip inlet 11 and a concentration gradient dilution channel 12; the height and width of the channel are 300 μm. The cell culture module 2 includes an open chamber 21 (3×4 array) downstream of the chip, a co...
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