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Application of catalpol in promoting differentiation of neural stem cells into oligodendrocytes

An oligodendrocyte and neural stem cell technology, applied in the field of stem cell therapy, can solve the problems of lack of control of NSCs differentiation and growth, unclear NSCs differentiation conditions, unclear regulatory mechanism, etc., achieving real-time observation, saving experimental time, The effect of generating drug concentration

Active Publication Date: 2018-04-20
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still many difficulties in the clinical application of NSCs in the current clinical treatment, due to the unclear differentiation conditions of NSCs, unclear regulatory mechanisms, lack of methods to control the differentiation and growth of NSCs after transplantation, etc.

Method used

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  • Application of catalpol in promoting differentiation of neural stem cells into oligodendrocytes
  • Application of catalpol in promoting differentiation of neural stem cells into oligodendrocytes
  • Application of catalpol in promoting differentiation of neural stem cells into oligodendrocytes

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Embodiment 1

[0032] Application of catalpol in promoting the differentiation of NSCs into oligodendrocytes.

[0033] 1 Design and fabrication of microfluidic chip

[0034] 1) For the study of the application of catalpol in promoting the differentiation of NSCs into oligodendrocytes, the schematic diagram of the microfluidic chip device used is as follows figure 1 shown. The whole device is composed of an upstream concentration gradient generator 1 and a downstream cell culture module 2 . Due to the ability to perform integrated operations in the chip (gradient concentration generation, stimulation and cell seeding, culturing, etc.), multiple cellular responses stimulated by different drug concentrations can be recorded simultaneously. The concentration gradient generator 1 includes a chip inlet 11 and a concentration gradient dilution channel 12; the height and width of the channel are 300 μm. The cell culture module 2 includes an open chamber 21 (3×4 array) downstream of the chip, a co...

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Abstract

The invention discloses an application of catalpol in promoting differentiation of neural stem cells (NSCs) into oligodendrocytes, and specifically, evaluates the effect of the natural active drug catalpol with different concentrations on differentiation of the NSCs into the oligodendrocytes by a drug concentration gradient micro-fluidic chip. Results indicate that the catalpol has no toxic effecton the NSCs at the concentration of lower than 3 mg / mL, and can obviously promote the differentiation of the NSCs into the oligodendrocytes. The system provides a platform for in-vitro studying of anaction mechanism of the traditional Chinese medicine active component catalpol on the differentiation of the NSCs to the oligodendrocytes. The promoting effect of the traditional Chinese medicine active component catalpol on the differentiation of the NSCs into the oligodendrocytes provides the basis and feasible technical support for development of NSCs replacement treatment for central nervoussystem demyelinating disease in preclinical phase.

Description

technical field [0001] The invention belongs to the evaluation and screening of related drugs in the field of stem cell therapy, and specifically relates to the application of catalpol in promoting the differentiation of neural stem cells into oligodendrocytes. Background technique [0002] Demyelinating diseases of the central nervous system are a group of diseases in which the central nervous system is dominated by extensive primary demyelination. The disease is caused by damage or developmental disorders of oligodendrocytes leading to damage or loss of myelin sheath, which affects the conduction of nerve impulses and makes nerve dysfunction. Oligodendrocytes, as the cells necessary for the formation of myelin in the central nervous system, can maintain the normal function and structure of the nervous system, and can also provide nerve growth factors and nutritional factors for the central nervous system. At present, most demyelinating diseases can only be treated symptom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/079C12M3/00C12M1/00
CPCC12M23/16C12N5/0622C12N2500/35C12N2506/08
Inventor 刘晶王亚辰马静云王亮
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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